Chemical apparatus and process disinfecting – deodorizing – preser – Chemical reactor – Organic polymerization
Patent
1995-07-20
1997-10-28
Kim, Christopher
Chemical apparatus and process disinfecting, deodorizing, preser
Chemical reactor
Organic polymerization
422 62, 422 81, 422110, 435 911, 935 88, 137561A, C08F 200
Patent
active
056815344
ABSTRACT:
The present invention relates to a high-throughput oligonucleotide synthesizer for synthesizing multiple oligonucleotides comprising a pre-existing low throughput oligonucleotide synthesizer which has been integrated with an increased throughput expansion module in order to increase the throughput capacity of the low throughput synthesizer. More specifically, the present invention comprises (a) a pre-existing low-throughput oligonucleotide synthesizer having at least one and no more than eight reactor positions, (b) a plurality of valve manifolds each having a single inlet port and a plurality of outlet ports, (c) a plurality of fluidic connection means each having a first end in fluid connection with one of said reactor positions of the pre-existing low-throughput oligonucleotide synthesizer and a second end in fluidic connection with the inlet port of one of the plurality of valve manifolds and (d) a plurality of reactor positions corresponding in number to the number of valve manifold outlet ports, each of the plurality of reactor positions (d) having a single reactor inlet such that each of said reactor inlets is in fluid connection with a corresponding valve manifold outlet port. Each of the plurality of reactor positions (d) is configured to accept a pre-packaged reactor column, such as a pre-packaged reactor column comprising a substrate having a first 3' base covalently attached thereto. Multiple oligonucleotides are synthesized by the high-throughput oligonucleotide synthesizer of the present invention by the delivery of a stream of reagents from the pre-existing low throughput synthesizer to the plurality of reactor positions (d). In this manner, the high-throughput oligonucleotide synthesizer increases the throughput capacity of a conventional oligonucleotide low throughput synthesizer having the standard 2 or 4 reactor positions to an automated operation of up to 64 reactor positions, and typically from 12 to 24 reactor positions.
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Borsari Peter A.
Kim Christopher
LandOfFree
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