High-throughput methods for detecting DNA methylation

Details High-throughput methods for detecting DNA methylation High-throughput methods for detecting DNA methylation

2006-12-05

2006-12-05

Goldberg, Jeanine A. (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S287200, C536S023100

Reexamination Certificate

active

07144701

ABSTRACT:
The present invention provides a method of hybridization, differential methylation hybridization (DMH) for high throughput methylation analysis of multiple CpG island loci. DMH utilizes nucleic acid probes prepared from a cell sample to screen numerous CpG dinucleotide rich fragments affixed on a screening array. Positive hybridization signals indicate the presence of methylated sites. Methods of preparing the hybridization probes and screening array are also provided.

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