High-throughput glutathione S-transferase polymorphic allele...

Details High-throughput glutathione S-transferase polymorphic allele... High-throughput glutathione S-transferase polymorphic allele...

2006-08-01

2006-08-01

Myers, Carla J. (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S023500, C536S024310, C536S024330

Reexamination Certificate

active

07083923

ABSTRACT:
A high-throughput assay for characterizing a subject's genetic makeup is disclosed. Specifically a high-throughput assay utilizing PCR is disclosed that permits the rapid and accurate characterization of a subject's inherited alleles of the polymorphic glutathione S-transferase (GST) genes GSTM1, GSTM3, GSTP1, and GSTT1. This method allows detection of the specific alleles inherited, including the gene dosage of GSTM1 and GSTT1 while not requiring restriction endonuclease digestion of the PCR products in order to detect length differences. Further, the method allows all analyses to be performed simultaneously in the same gel lane, thus further adding efficiency and cost-effectiveness.

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“High-Throughput Genotyping Method for Glutathione,”Research Communications in Molecular Pathology and Pharmacology, vol. 103, No. 1, Michael M. Shi, pp. 3-15.

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