High throughput gene inactivation with large scale gene targetin

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

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435471, 435354, 435325, 435243, 43525233, 435 6, 800 18, 800 21, 800 22, 800 25, C12N 1500, C12N 1563, C12N 1509, C12N 500

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060690100

ABSTRACT:
The present invention provides a vector system that is useful for the generation of mutations in a recombination-based construction method. The invention further includes the incorporation of mutations generated by the method of the present invention into mouse embryonic stem cells and transgenic mice.

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