High-throughput, dual probe biological assays based on...

Details High-throughput, dual probe biological assays based on... High-throughput, dual probe biological assays based on...

2006-07-11

2006-07-11

Wu, Jingge (Department: 2623)

Image analysis

Applications

Biomedical applications

Reexamination Certificate

active

07076092

ABSTRACT:
A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0.5 μm×100 μm×100 μm. A device using these methods would have 103–104greater throughput than previous one-dimensional (1D) micro-stream devices having 1 μm3interrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (˜10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

REFERENCES:
patent: 4979824 (1990-12-01), Mathies et al.
patent: 5961924 (1999-10-01), Reichert et al.
patent: 6608918 (2003-08-01), Rushbrooke et al.
patent: WO 99/40416 (1999-08-01), None
Princeton Instruments Datasheet for Pentamax-512EFT camera, p. 28, http://depts.washington.edu/keck/512eft.pdf.
W. Trabesinger et al, Analytical Chemistry, 2001, 73, 110-1105.
Y. Ma et al. Analytical Chemistry, 2000, 72, 4640-4645.
W.A. Lyon et al, Analytical Chemistry, 1997, 69, 3400-3405.
F. Loscher et al. Analytical Chemistry, 1998, 70, 3202-3205.
C. Zander et al, Chemical Physics Letters, 286 (1998) 457-465.

Affiliated with

Also associated with

Rating

High-throughput, dual probe biological assays based on... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High-throughput, dual probe biological assays based on..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High-throughput, dual probe biological assays based on... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3529924