High throughput DNA sequencing vector

Details High throughput DNA sequencing vector High throughput DNA sequencing vector

1998-04-10

2000-02-08

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 435 915, 4353201, 435476, 536 232, 536 237, 536 241, 536 2433, C12N 938, C12N 1511, C12N 1564, C12Q 100, C12Q 104

Patent

active

060227168

ABSTRACT:
High throughput DNA sequencing vectors for generating nested deletions using enzymatic techniques and/or transposition-based techniques are disclosed. Methods of constructing contigs of long DNA sequences and methods of generating nested deletions are also disclosed. A truncated lacZ derivative useful in measuring the copy number of the lacZ derivative in a host cell is also disclosed.

REFERENCES:
Ahmed, A., "Use of transposon-promoted deletions in DNA sequence analysis", J. Mol. Biol. 174(4): 941-948 (1984).
Chen, E., et al., J. Genomics 17: 651-656 (1993).
Guo, L.H., et al., Nucleic Acids Research 11(6): 5521-5540 (1983).
Christyakov, D.A., et al., "New cosmid vector pDEL-BAC: cloning of large fragments of genomic DNA for subsequent introduction of deletions and sequencing of the DNA of the deletion mutants which are formed", Molecular Biology 30(3): 1-20 (1996). (English translation of #4 above).
Henikoff, S., "An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid", Gene 28: 351-359 (1984).
Roach, J.C., et al., :Pairwise end sequencing: a unified approach to genomic mapping and sequencing, Genomics 26: 345-353 (1995).
Staden, R.A., "A new computer method for the storage and manipulation of DNA gel reading data", Nucleic Acids Research 8: 3673-3694 (1980).
Wang, G., et al., "pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo", Proc. Natl. Acad. Sci. USA 90(16): 7874-7878 (1983).
GenBank Accession No. M77789, date Oct. 23, 1991.
GenBank Accession No. J01688, date Oct. 28, 1994.
GenBank Accession No. X06404, date Nov. 8, 1995.
GenBank Accession No. U46017, date Feb. 12, 1996.
GenBank Accession No. U51113, date Feb. 6, 1997.

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