Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues
Reexamination Certificate
2007-01-02
2007-01-02
Kam, Chih-Min (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
C530S324000, C530S333000, C530S344000, C530S350000, C435S004000, C435S007710, C435S007720, C435S219000
Reexamination Certificate
active
11195098
ABSTRACT:
In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can serve as immobilized substrates. In both types a fluorescent molecules is present in the substrate, eliminating the requirement for the addition of a fluorigenic reagent. The assays described can be readily adapted for use in automated or robotic systems.
REFERENCES:
patent: 5605809 (1997-02-01), Komoriya et al.
patent: 5965699 (1999-10-01), Schmidt et al.
patent: 6762280 (2004-07-01), Schmidt et al.
patent: WO 95/33850 (1995-12-01), None
patent: WO 0192312 (2001-12-01), None
Soleilhac et al. (1996) A sensitive and rapid fluoresence-based assay for determination of tetanus toxin peptidase activity. Analytical Biochemistry 241, 120-127.
Anne. C. et al. (2001) High-throughput fluorogenic assay for determination of botulinum type B neurotoxin protease activity. Analytical Biochemistry 291, 253-261.
Roques, B. P. et al. (2000) Mechanism of action of clostridial neurotoxins and rational inhibitor design. Biology of the Cell 92, 445-557.
Schmidt James J.
Stafford Robert G.
Arwine Elizabeth
Kam Chih-Min
The United States of America as represented by the Secretary of
LandOfFree
High throughput assays for the proteolytic activities of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High throughput assays for the proteolytic activities of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High throughput assays for the proteolytic activities of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3787319