High throughput assays for the proteolytic activities of...

Details High throughput assays for the proteolytic activities of... High throughput assays for the proteolytic activities of...

2006-04-25

2006-04-25

Kerr, Kathleen M. (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

C530S324000, C530S333000, C530S344000, C530S350000, C424S239100, C435S004000, C435S007710, C435S007720, C435S219000

Reexamination Certificate

active

07034107

ABSTRACT:
In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can serve as immobilized substrates. In both types a fluorescent molecules is present in the substrate, eliminating the requirement for the addition of a fluorigenic reagent. The assays described can be readily adapted for use in automated or robotic systems.

REFERENCES:
patent: 5605809 (1997-02-01), Packard et al.
patent: 5965699 (1999-10-01), Bostian et al.
patent: WO 95/33850 (1995-12-01), None
patent: WO0192312 (2001-12-01), None
Soleilhac et al. (1996) A sensitive and rapid fluoresence-based assay for determination of tetanus toxin peptidase activity. Analytical Biochemistry 241, 120-127.
Anne, C. et al. (2001) High-throughput fluorogenic assay for determination of botulinum type B neurotoxin protease activity. Analytical Biochemistry 291, 253-261.
Roques, B. P. et al. (2000) Mechanism of action of clostridial neurotoxins and rational inhibitor design. Biology of the Cell 92, 445-557.

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