High throughput assay using fusion proteins

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 75, 435 772, 435 78, 435 697, 435 712, 4353201, 530324, 530344, 530350, 530415, 536 234, 536 241, G01N 3353, G01N 33544, G01N 33566, G01N 3368

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057833982

ABSTRACT:
This application describes a high throughput assay for screening for compounds capable of binding to a fusion protein which consists of a target protein and an FK506-binding protein. The method for preparing the DNA encoding for the fusion protein and for expressing that DNA is also described in the application. The invention also discloses the recombinant DNA and protein sequences for several fusion proteins.

REFERENCES:
patent: 5279957 (1994-01-01), Gross
patent: 5352660 (1994-10-01), Pawson
patent: 5434064 (1995-07-01), Schlessinger et al.
patent: 5464745 (1995-11-01), Mierendorf et al.
patent: 5498538 (1996-03-01), Kay et al.
patent: 5498597 (1996-03-01), Burakoff et al.
patent: 5534424 (1996-07-01), Uhlen et al.
patent: 5580979 (1996-12-01), Bachovchin
Edalji et al. High-level expression of recombinant human FK-binding protein from a fusion precursor. Journal of Protein Chemistry. vol. 11, No. 3, pp. 213-223, 1992.
Gilmer et al. Peptide inhibitors of src SH3-SH2-phosphoprotein interactions. The Journal of Biological Chemistry. vol. 269, No. 50, pp. 31711-31719, Dec. 16, 1994.
Jin et al. The 25-kDa FK506-binding protein is localized in the nucleus and associates with casein Kinase II and nucleolin. Proceedings of the National Academy of Sciences, USA, vol. 90, pp. 7769-7773, Aug. 1993.
Muller et al. Rapid identification of phosphopeptide ligands for SH2 domains: screening of peptide libraries by fluorescence-activated bead sorting. The Journal of Biological Chemistry. vol. 271, No. 28, pp. 16500-16505, Jul. 12, 1996.
Sampson et al. Neisseria meningitidis encodes an FK506-inhibitable rotamase. Proceedings of the National Academy of Sciences, USA. vol. 89, pp. 1164-1168, Feb. 1992.
Studier et al. Use of T7 RNA polymerase to direct expression of cloned genes. In: Methods in Enzymology, Academic Press, SanDiego, CA. vol. 185, pp. 60-89, 1990.
Lemmon, et al., "Thermodynamic Studies of Tyrosyl-Phosphopeptide Binding to the SH2 Domain of p56lck", Biochemistry, vol. 33, pp. 5070-5076, 1994.
Payne, et al., "Kinetics of p56lck and p60src Src Homology 2 Domain Binding to Tyrosine-Phosphorylated Peptides Determined by a Competition Assay or Surface Plasmon Resonance", Proc. Natl. Acad. Sci. USA, vol. 90, pp. 4902-4906, 1993.
Isakov, et al., ZAP-70 Binding Specificity to T Cell Receptor Tyrosine-Based Activation Motifs: The Tandem SH2 Domains of ZAP-70 Blind Distinct Tyrosine-Based Activation Motifs with Varying Affinity, J. Exp. Med., vol. 181, pp. 375-380, 1995.
Huyer, et al., "Direct Determination of the Sequence Recognition Requirements of the SH2 Domains of SH-PTP2", Biochemistry, vol. 34, pp. 1040-1049, 1995.
Panayotou, et al., "Interactions Between SH2 Domains and Tyrosine-Phosphorylated Platelet-Derived Growth Factor Beta-Receptor Sequences: Analysis of Kinetic Parameters by a Novel Biosensor-Based Approach", Mol. Cell. Biol., vol. 13, pp. 3567-3576, 1993.
Felder, et al., "SH2 Domains Exhibit High-Affinity Binding to Tyrosine-Phosphorylated Peptides Yet Also Exhibit Rapid Dissociation and Exchange", Mol. Cell. Biol., vol. 13, pp. 1449-1455, 1993.
Morelock, et al., "Determination of Receptor-Ligand Kinetic and Equilibrium Binding Constants Using Surface Plasmon Resonance: Application to the lck SH2 Domain and Phosphotyrosyl Peptides", J. Med. Chem., vol. 38, pp. 1309-1318, 1995.
Ladbury, et al., "Measurement of the Binding of Tyrosyl Phosphopeptides to SH2 Domains: A Reappraisal", Proc. Natl. Acad. Sci. USA, vol. 92, pp. 3199-3203, 1995.
Piccione, et al., "Phosphatidylinositol 3-Kinase p85 SH2 Domain Specificity of Defined by Direct Phosphopeptide/SH2 Domain Binding", Biochemistry, vol. 32(13), pp. 3197-3202, 1993.
Sonatore, et al., "The Utility of FK506-Binding Protein as a Fusion Partner in Scintillation Proximity Assays: Application to SH2 Domains", Anal. Biochemistry, vol. 240, pp. 289-297, 1996.

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