Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-09-04
1998-07-07
Kemmerer, Elizabeth C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435 772, 435 78, 530324, 530344, 530350, 536 234, G01N 3353, G01N 33546, G01N 33566, G01N 3360, G01N 3368
Patent
active
057766967
ABSTRACT:
This application describes a high throughput assay for screening for compounds which are capable of binding to a fusion protein which consists of a target protein and an FKS506-binding protein.
REFERENCES:
patent: 5352660 (1994-10-01), Pawson
patent: 5498597 (1996-03-01), Burakoff et al.
patent: 5580979 (1996-12-01), Bachovchin
Edalji et al. High-level expression of recombinant human FK-binding protein from a fusion precursor. Journal of Protein Chemistry. vol. 11, No. 3, pp. 213-223, 1992.
Gilmer et al. Peptide inhibitors of src SH3-SH2-phosphoprotein interactions. The Journal of Biological Chemistry. vol. 269, No. 50, pp. 31711-31719, Dec. 16, 1994.
Jin et al. The 25-kDa FK506-binding protein is localized in the nucleus and associates with casein kinase II and nucleolin. Proceedings of the National Academy of Sciences, USA, vol. 90, pp. 7769-7773, Aug. 1993.
Muller et al. Rapid identification of phosphopeptide ligands for SH2 domains: screening of peptide libraries by fluorescence-activated bead sorting. The Journal of Biological Chemistry. vol. 271, No. 28, pp. 16500-16505, Jul. 12, 1996.
Sampson et al. Neisseria meningitidis encodes an FK506-inhibitable rotamase. Proceedings of the National Academy of Sciences, USA. vol. 89, pp. 1164-1168, Feb. 1992.
Lerner, et al., "Scintillation Proximity Assay for Human DNA Topoisomerase I Using Recombinant Biotinyl-Fusion Protein Produced in Baculovirus-Infected Insect Cells", Anal. Biochem., vol. 240, pp. 289-297 (1996).
Skinner, et al., "Direct Measurment ofthe Binding of RAS to Neurofibromin Using a Scintillation Proximity Assay", Anal. Biochem., vol. 233, pp. 259-265, (1994).
Lemmon, et al., "Thermodynamic Studies of Tyrosyl-Phosphopeptide Binding to the SH2 Domain of p56Ick", Biochemistry, vol. 33, pp. 5070-5076, 1994.
Payne, et al., "Kinetics of p56Ick and p60src Homology 2 Domain Binding to Tyrosine-Phosphorylated Peptides Determined by a Competition Assay or Surface Plasmon Resonance", Proc. Natl. Acad. Sci. USA, vol. 90, pp. 4902-4906, 1993.
Isakov, et al., ZAP-70 Binding Specificity to T Cell Receptor Tyrosine-Based Activation Motifs: The Tandem SH2 Domains of ZAP-70 Bind District Tyrosine-Based Activation Motifs with Varying Affinity, J. Exp. Med., vol. 181, pp. 375-380, 1995.
Huyer, et al., "Direct Determination of the Sequence Recognition Requirements of the SH2 Domains of SH-PTP2", Biochemistry, vol. 34, pp. 1040-1049, 1995.
Panayotou, et al., "Interactions Between SH2 Domains and Tyrosine-Phosphorylated Platelet-Derived Growth Factor Beta-Receptor Sequences: Analysis of Kinetic Parameters by a Novel Biosenor-Based Approach", Mol. Cell. Biol., vol. 13, pp. 3567-3576, 1993.
Felder, et al., "SH2 Domains Exhibit High-Affinity Binding to Tyrosine-Phosphorylated Peptides Yet Also Exhibit Rapid Dissociation and Exchange", Mol. Cell. Biol., vol. 13, pp. 1449-1455, 1993.
Morelock, et al., "Determination of Receptor-Ligand Kinetic and Equilibrium Binding Constants Using Surface Plasmon Resonance: Application to the Ick SH2 Domain and Phosphotyrosyl Peptides", J. Med. Chem., vol. 38, pp. 1309-1318, 1995.
Ladbury, et al., "Measurement of the Binding of Tyrosyl Phosphopeptides to SH2 Domains: A Reappraisal", Proc. Natl. Acad. Sci. USA, vol. 92, pp. 3199-323, 1995.
Piccione, et al., "Phosphatidylinositol 3-Kinase p85 SH2 Domain Specificity Defined by Direct Phosphopeptide/SH2 Domain Binding", Biochemistry, vol. 32(13), pp. 3197-3202, 1993.
Sonatore, et al., "The Utility of FK506-Binding Protein as a Fusion Partner in Scintillation Proximity Assays: Appliction to SH2 Domains", Anal. Biochemistry, vol. 240, pp. 289-297, 1996.
Camara Valerie J.
Daniel Mark R.
Kemmerer Elizabeth C.
Lathrop Brian
Merck & Co. , Inc.
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