High-throughput assay for virus entry and drug screening

Details High-throughput assay for virus entry and drug screening High-throughput assay for virus entry and drug screening

2008-05-05

2010-10-05

Mosher, Mary E (Department: 1648)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

C435S007100, C435S007900, C435S029000, C435S235100, C435S975000

Reexamination Certificate

active

07807346

ABSTRACT:
The present invention provides a rapid virus entry/binding detection assay. An enzyme such as luciferase was incorporated at the C-terminal end of viral envelope proteins of the HIV Nef protein that would specifically associate with cell membranes to deliver the enzyme into viral particles upon viral assembly. Virus entry/binding can then be assayed by determining the enzymatic activities in infected cells. The assay allows high-throughput non-radioactive detection of virus entry within 30 minutes after virus-cell contact. This assay provides high signal to noise ratio and is useful for screening compounds that affect virus-cell binding and entry. The design also permits packaging of potential therapeutic proteins into functional virus particles and delivering them to specific cellular targets.

REFERENCES:
patent: 2001/0048928 (2001-12-01), Joseph O'Hare et al.
patent: 2004/0096823 (2004-05-01), Greene et al.
Forshey et al (Journal of Virology 77:4409-4414, 2003).
Kolokoltsov et al (Virology 347:333-342, 2006).
Saeed et al (Journal of Virological Methods 135:143-150, 2006.
Brignati et al (Journal of Virology 77:4888-4898, 2003).

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