Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-06-03
1999-03-02
Stucker, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 771, 435 772, 435 79, 435 792, 435 793, 436501, 436518, 436531, 436534, 2502612, G01N 3353
Patent
active
058769463
ABSTRACT:
A homogeneous high throughput assay is described which screens compounds for enzyme inhibition, or receptor or other target binding. Inhibition (or binding) by the library compounds causes a change in the amount of an optically detectable label that is bound to suspendable cells or solid supports. The amounts of label bound to individual cells or solid supports are microscopically determined, and compared with the amount of label that is not bound to individual cells or solid supports. The degree of inhibition or binding is determined using this data. Confocal microscopy, and subsequent data analysis, allow the assay to be carried out without any separation step, and provide for high throughput screening of very small assay volumes using very small amounts of test compound.
REFERENCES:
patent: 4568649 (1986-02-01), Bertoglio-Matte
patent: 5032720 (1991-07-01), White
patent: 5091652 (1992-02-01), Mathies et al.
patent: 5114477 (1992-05-01), Mort et al.
patent: 5120953 (1992-06-01), Harris
patent: 5162941 (1992-11-01), Favro et al.
patent: 5210636 (1993-05-01), Baer
patent: 5260578 (1993-11-01), Bliton et al.
patent: 5274240 (1993-12-01), Mathies et al.
patent: 5283684 (1994-02-01), Thomas et al.
patent: 5304810 (1994-04-01), Amos
patent: 5313079 (1994-05-01), Brasen et al.
patent: 5323079 (1994-06-01), Nieves et al.
patent: 5334870 (1994-08-01), Katada et al.
patent: 5351152 (1994-09-01), Kuo et al.
patent: 5547849 (1996-08-01), Baer et al.
patent: 5556764 (1996-09-01), Sizto et al.
Molecular Devices, Fluorescent Imaging Plate Reader, the Premier Optical Screening Instrument, 4 pages.
Dietz et al., Volumetric Capillary Cytometry: A New Method for Absolute Cell Enumeration, Cytometry 23:177-186 (1996).
Quesada et al., Chemosensitization and drug accumulation assays as complementary methods for the screening of multidrug resistance reversal agents, Cancer Letters 99 (1996) 109-114.
Manz et al., In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy, Microbiology (1995), 141, 29-39.
Michael Hanley, Peptide Binding Assays, Neurotransmitters Receptor Binding, 2nd edition, 1985, pp. 91-102.
Schroeder et al., FLIPR: A New Instrument for Accurate, High Throughput Optical Screening, Journal of Biomolecular Screening, vol. 1, No. 2, 1996, pp. 75-80.
Cuatrecasas et al., Membrane Receptors and Hormone Aciton, Advances in Protein Chemistry 30,251-451 (1976).
Ted Dixon, Random mask brightens image, Nature, vol. 383, Oct. 31, 1996, pp. 760-761.
Sales Brochure, IMAGN 2000, 4 pages.
Morrow et al. "Synthetic Peptides from a Conserved Region of the HIV Envelope Induce Broadly Reactive Anti-HIV Responses" International Conference on AIDS, vol. 6, No. 1 (1990), meeting abstract, p. 207 (Abstract No. Th.A.350).
Burbaum Jonathan J.
Chelsky Daniel
Chung Thomas D.Y.
Inglese James
Kirk Gregory L.
Pharmacopeia Inc.
Stucker Jeffrey
LandOfFree
High-throughput assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High-throughput assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High-throughput assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-421407