Liquid purification or separation – With means to add treating material – Chromatography
Patent
1999-04-19
2000-07-18
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
210656, 210659, 73 6152, 422 70, B01D 1508
Patent
active
060902809
ABSTRACT:
A method and apparatus for high-throughput identity confirmation, quantitation and purity determination of a compound of interest in a mixture of compounds is disclosed. According to the method, a chemical mixture, such as may be obtained from a chemical library, is processed to obtain a stream having a time-varying composition, such as by using a high pressure liquid chromatograph. The stream is split into two portions or streams for substantially contemporaneous detection in a compound-identifying device, such as a mass spectrograph, and in a compound-quantitating device, such as an evaporative light scattering detector. A peak in a chromatogram obtained from the compound-quantitating device is identified as the compound of interest by cross-correlation, via elution time, with chromatographic results obtained from the compound-identifying device. Once the peak is identified as corresponding to the compound of interest, the corresponding peak area is used for quantitation and purity determination.
REFERENCES:
patent: 4840730 (1989-06-01), Saxena
patent: 5234586 (1993-08-01), Afeyan
patent: 5766481 (1998-06-01), Zambias
patent: 5827946 (1998-10-01), Klee
Connelly James A.
O'Brien Linda
Zhang Sue Xiuqing
Zhao Ning
Breyer Wayne S.
DeMont Jason P.
Pharmacopeia Inc.
Therkorn Ernest G.
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