Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-02-20
2007-02-20
Goldberg, Jeanine A. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C435S091210, C536S023100
Reexamination Certificate
active
09823649
ABSTRACT:
The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.
REFERENCES:
patent: 5310652 (1994-05-01), Gelfand et al.
patent: 5374553 (1994-12-01), Gelfand et al.
patent: 5561058 (1996-10-01), Gelfand et al.
patent: 5614365 (1997-03-01), Tabor et al.
patent: 5830714 (1998-11-01), Swaminathan et al.
patent: 5968799 (1999-10-01), Gelfand et al.
patent: 6015668 (2000-01-01), Hughes et al.
patent: 6083686 (2000-07-01), Sullivan
patent: 6346379 (2002-02-01), Gelfand et al.
patent: 0 482 714 (1992-04-01), None
patent: 0 655 506 (1995-05-01), None
patent: 0 823 479 (1998-02-01), None
patent: 0 902 035 (1999-03-01), None
patent: 0 902 035 (1999-06-01), None
patent: WO 92/06202 (1992-04-01), None
patent: WO 95/14770 (1994-11-01), None
patent: WO 95/14770 (1995-06-01), None
patent: WO 98/40496 (1998-09-01), None
patent: WO 00/71739 (2000-11-01), None
Kawasaki. “Amplfiication of RNA” PCR Protocols, Chapter 3, pp. 21-28, 1990.
Maniatis et al. “Molecular Cloning.” 1983, pp. 213-215.
Myers, T. W. et al, “Amplification of RNA: High Temperature Reverse Transcription and DNA Amplification With Thermus Thermophilus DNA Polymerase”,PCR Strategies, 1995, Chapter 5: 58-68.
Selner, Loryn and Turbett, Gavin, “Comparison of Three RT-PCR Methods, ” BioTechniques (1998) 25:230-234 (Royal Perth Hospital).
Tse, William T., et al., “Reverse Transcription and Direct Amplification of Cellular RNA Transcripts by TaqPolymerase, ” Gene (1990) 88:293-296 (0378-1119/90).
Beckman, Robert A., et al., “On the Fidelity of DNA Replication: Manganese Mutagenesis in Vitro, ” Biochemistry (1985) 24:5810-5817 (0006-2960/85).
Leung, David W., et al., “A Method for Random Mutagenesis of a Defined DNA Segment Using a Modified Polymerase Chain Reaction,” Technique (1989) vol. 1, 1:11-15 (1043-4658/89).
Brown, D.M., “Basic Principles in Nucleic Acid Chemistry—Chemical Reactions of Polynucleotides and Nucleic Acids,” Academic Press, (1974) vol. II, 1-90 (ISBN 0-12-701902-2(v. 2)).
Sellner, et al., 1998, “Comparison of Three RT-PCR Methods.”Biotechniques, 25(2): 230-234.
Abramson, Richard D.; “Thermostable DNA Polymerases”; 1999,PCR Strategies, pp. 39-57.
Abramson, Richard D.; “Thermostable DNA Polymerases: An Update”; 1999,PCR Applications: Protocols for Functional Genomics, pp. 33-48.
Landre, P.A. et al.; “The Use of Cosolvents to Enhance Amplification by the Polymerase Chain Reaction”; 1989,PCR Strategies, pp. 3-16.
Lawyer, Frances C. et al.; “Isolation, Characterization, and Expression inEscherichia coliof the DNA Polymerase Gene fromThermus aquaticus”; 1989,The Journal of Biological Chemistry, vol. 264, No. 11, pp. 6427-6437.
Reeve, et al., “A Novel Thermostable Polymerase for DNA Sequencing,”Nature, 1995, vol. 376, pp. 796-797.
Elfstrom Carita Maria
Gelfand David Harrow
Higuchi Russell Gene
Myers Thomas William
Schönbrunner Nancy Jeneane
Doyle Charles M.
Goldberg Jeanine A.
Roche Molecular Systems, Inc
Sappenfiel Christopher C.
Townsend & Townsend & Crew LLP
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