Liquid purification or separation – With means to add treating material – Chromatography
Reexamination Certificate
2005-01-25
2005-01-25
Therkorn, Ernest G. (Department: 1723)
Liquid purification or separation
With means to add treating material
Chromatography
C210S502100, C210S635000, C210S656000, C502S401000
Reexamination Certificate
active
06846410
ABSTRACT:
The present invention describes the use of porous metal oxides for the preparative purification of antibodies and biomolecules within a range of particle physical characteristics. Typically, particles from 15 to 100 microns in average diameter with pores sizes ranging from 400 to 600 angstroms and surface areas from 10 to 50 square meters per gram, and pore volumes from 0.1 to 0.4 mL/gram can be used for purification processes. The metal oxide particles that fall within this range of physical properties show enhanced utility and greater chromatographic capacity for antibodies than materials oxide particles falling outside of this range. Metal oxides such as zirconia, titania and alumina can all be modified with a multi-Lewis base moiety such as an organophosphate ethylenediamine-N,N-tetra(methylenephosphonic) acid (EDTPA), to produce a bio-compatible purification media for, biomolecules.
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Carr Peter W.
Hawker Danielle R.
Johnson Kerry G.
McNeff Clayton V.
Rupp Steven J.
Merchant & Gould P.C.
Therkorn Ernest G.
ZirChrom Separations, Inc.
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