High speed nucleic acid sequencing

Details High speed nucleic acid sequencing High speed nucleic acid sequencing

2011-05-17

2011-05-17

Sisson, Bradley L (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S183000, C435S287200, C536S023100, C536S024330

Reexamination Certificate

active

07943305

ABSTRACT:
The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.

REFERENCES:
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 5079169 (1992-01-01), Chu et al.
patent: 5200313 (1993-04-01), Carrico
patent: 5302509 (1994-04-01), Cheeseman
patent: 5403708 (1995-04-01), Brennan et al.
patent: 5405747 (1995-04-01), Jett et al.
patent: 5470710 (1995-11-01), Weiss et al.
patent: 5545522 (1996-08-01), Van Gelder et al.
patent: 5547835 (1996-08-01), Koster
patent: 5547839 (1996-08-01), Dower et al.
patent: 5601982 (1997-02-01), Sargent et al.
patent: 5620854 (1997-04-01), Holzrichter et al.
patent: 5631134 (1997-05-01), Cantor
patent: 5646264 (1997-07-01), Glazer et al.
patent: 5661028 (1997-08-01), Foote
patent: 5677196 (1997-10-01), Herron et al.
patent: 5679524 (1997-10-01), Nikiforov et al.
patent: 5681699 (1997-10-01), Rotter et al.
patent: 5688648 (1997-11-01), Mathies et al.
patent: 5695934 (1997-12-01), Brenner
patent: 5703222 (1997-12-01), Grossman et al.
patent: 5723591 (1998-03-01), Livak et al.
patent: 5763181 (1998-06-01), Han et al.
patent: 5773308 (1998-06-01), Conrad et al.
patent: 5831030 (1998-11-01), Tsujimoto et al.
patent: 5846727 (1998-12-01), Soper et al.
patent: 5858671 (1999-01-01), Jones
patent: 5877015 (1999-03-01), Hardy et al.
patent: 5922591 (1999-07-01), Anderson et al.
patent: 5961923 (1999-10-01), Nova et al.
patent: 5976802 (1999-11-01), Ansorge et al.
patent: 6004744 (1999-12-01), Goelet et al.
patent: 6027890 (2000-02-01), Ness et al.
patent: 6048690 (2000-04-01), Heller et al.
patent: 6210896 (2001-04-01), Chan
patent: 6221592 (2001-04-01), Schwartz et al.
patent: 6232075 (2001-05-01), Williams
patent: 6255083 (2001-07-01), Williams
patent: 6263286 (2001-07-01), Gilmanshin et al.
patent: 6280939 (2001-08-01), Allen
patent: 6306607 (2001-10-01), Williams
patent: 6355420 (2002-03-01), Chan
patent: 6399335 (2002-06-01), Kao et al.
patent: 6403311 (2002-06-01), Chan
patent: 6485944 (2002-11-01), Church et al.
patent: 6524829 (2003-02-01), Seeger
patent: 6632655 (2003-10-01), Mehta et al.
patent: 6762048 (2004-07-01), Williams
patent: 6780591 (2004-08-01), Williams et al.
patent: 6936702 (2005-08-01), Williams et al.
patent: 7008766 (2006-03-01), Densham
patent: 7033764 (2006-04-01), Korlach et al.
patent: 7052847 (2006-05-01), Korlach et al.
patent: 7056661 (2006-06-01), Korlach et al.
patent: 7056676 (2006-06-01), Korlach et al.
patent: 7229799 (2007-06-01), Williams
patent: 7244566 (2007-07-01), Sood et al.
patent: 7361466 (2008-04-01), Korlach et al.
patent: 7416844 (2008-08-01), Korlach et al.
patent: 7485424 (2009-02-01), Korlach et al.
patent: 2001/0014850 (2001-08-01), Gilmanshin et al.
patent: 2002/0025529 (2002-02-01), Quake et al.
patent: 2002/0119455 (2002-08-01), Chan
patent: 2002/0164629 (2002-11-01), Quake et al.
patent: 2003/0064366 (2003-04-01), Hardin et al.
patent: 2003/0138809 (2003-07-01), Williams et al.
patent: 2003/0186255 (2003-10-01), Williams et al.
patent: 2003/0194740 (2003-10-01), Williams et al.
patent: 2005/0260614 (2005-11-01), Hardin et al.
patent: 2006/0286570 (2006-12-01), Rowlen et al.
patent: 2007/0009954 (2007-01-01), Wang et al.
patent: 2007/0031829 (2007-02-01), Yasuno et al.
patent: 2007/0042400 (2007-02-01), Choi et al.
patent: 2007/0042419 (2007-02-01), Barany et al.
patent: 2008/0227654 (2008-09-01), Korlach et al.
patent: 0 745 686 (1996-12-01), None
patent: 0 258 017 (1997-06-01), None
patent: 0 834 576 (1998-04-01), None
patent: WO 90/13666 (1990-11-01), None
patent: WO 91/13075 (1991-09-01), None
patent: WO 93/21340 (1993-10-01), None
patent: WO 95/06138 (1995-03-01), None
patent: WO 96/27025 (1996-09-01), None
patent: WO 97/05156 (1997-02-01), None
patent: WO 98/28440 (1998-07-01), None
patent: WO 98/44152 (1998-10-01), None
patent: WO 98/45481 (1998-10-01), None
patent: WO 99/05315 (1999-02-01), None
patent: WO 99/19341 (1999-04-01), None
patent: WO 99/19516 (1999-04-01), None
patent: WO 00/06770 (2000-02-01), None
patent: WO 00/09757 (2000-02-01), None
patent: WO 00/36151 (2000-06-01), None
patent: WO 00/36152 (2000-06-01), None
patent: WO 00/40750 (2000-07-01), None
patent: WO 00/53805 (2000-09-01), None
patent: WO 00/53812 (2000-09-01), None
patent: WO 00/58507 (2000-10-01), None
patent: WO 00/60072 (2000-10-01), None
patent: WO 00/60114 (2000-10-01), None
patent: WO 01/13088 (2001-02-01), None
patent: WO 01/16375 (2001-03-01), None
patent: WO 01/23610 (2001-04-01), None
patent: WO 01/25480 (2001-04-01), None
patent: WO 01/32930 (2001-05-01), None
patent: WO 01/57248 (2001-08-01), None
patent: WO 01/57249 (2001-08-01), None
patent: WO 01/94609 (2001-12-01), None
patent: WO 02/02813 (2002-01-01), None
patent: WO 02/03305 (2002-01-01), None
patent: WO 02/29106 (2002-04-01), None
patent: WO 02/061126 (2002-08-01), None
patent: WO 02/061127 (2002-08-01), None
patent: WO 02/072892 (2002-09-01), None
patent: WO 02/095070 (2002-11-01), None
patent: WO 02/101095 (2002-12-01), None
patent: WO 03/016565 (2003-02-01), None
patent: WO 03/020734 (2003-03-01), None
Zhang, et al. Reconstruction of DNA sequencing by hybridization. Bioinformatics. Jan. 2003;19(1):14-21.
Document received from Robert W. Strozier, P.L.L.C. dated Sep. 25, 2007.
Alexandrova, et al. 2′-Deoxynucleoside 5′-triphosphates modified at alpha-, beta- and gamma-phosphates as substrates for DNA polymerases. Nucleic Acids Res. Feb. 1, 1998;26(3):778-86.
Chatterji et al. Fluorescence spectroscopy analysis of active and regulatory sites of RNA polymerase. Methods Enzymol. 1996;274:456-78.
Nyren, et al. Solid phase DNA minisequencing by an enzymatic luminometric inorganic pyrophosphate detection assay. Anal Biochem. Jan. 1993;208(1):171-5.
Schlageck, et al. Spectroscopic techniques for study of phosphodiester bond formation byEscherichia coliRNA polymerase. J Biol Chem. Dec. 10, 1979;254(23):12074-7.
Yarbrough, et al. Synthesis and properties of fluorescent nucleotide substrates for DNA-dependent RNA polymerases. J Biol Chem. Dec. 10, 1979;254(23):12069-73.
Davis, L.M. et al., “Rapid DNA Sequencing Based Upon Single Molecule Detection” (1991)Genetic Analysis Techniques and Applications, 8(1):1-7.
Dobrikov, M.I., et al., “Sensitized Photomodification of Single-Stranded DNA by a Binary System of Oligonucleotide Conjugates” (1997)Antisense Nucleic Acid Drug Development, 7(4):309-317.
Doore, K., et al., “Techniques for Single Molecule Sequencing” (1997) vol. 5: 139-152.
Harding, J.D., et al., “Single-Molecule Detection as an Approach to Rapid DNA Sequencing” (1992)Trends in Biotechnol., 10(1-2): 55-57.
Kawata, Y., et al., “Feasibility of Molecular-Resolution Fluorescence Near-Field Microscopy Using Multi-Photon Absorption and Field Enhancement Near a Sharp Tip” (1999)Journal of Applied Physics85(3): 1294-1301.
Kristensen, T., et al., “Rapid and Simple Preparation of Plasmids Suitable for Dideoxy DNA Sequencing and Other Purposes” (1991)DNA Seq.1(4): 227-232.
Nickerson, D.A., et al., “PolyPhred: Automating the Detection and Genotyping of Single Nucleotide Substitutions Using Fluorescence-Based Resequencing” (1997)Nucleic Acids Res., 25(14): 2745-2751.
Novotny, L. et al., “Theory of Nanometric Optical Tweezers” (1997)Physical Review Letters79(4):645-648.
Sanchez, E.J., et al., “Near-Field Fluorescence Microscopy Based on Two-Photon Excitation with Metal Tip

Affiliated with

Also associated with

Rating

High speed nucleic acid sequencing does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High speed nucleic acid sequencing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High speed nucleic acid sequencing will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2622277