High speed flow cytometric separation of viable cells

Details High speed flow cytometric separation of viable cells High speed flow cytometric separation of viable cells

1994-04-25

1995-11-14

Parr, Margaret

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

436 63, 436172, A01N 102

Patent

active

054665728

ABSTRACT:
Hematopoietic cell populations are separated to provide cell sets and subsets as viable cells with high purity and high yields, based on the number of original cells present in the mixture. High-speed flow cytometry is employed using light characteristics of the cells to separate the cells, where high flow speeds are used to reduce the sorting time.

REFERENCES:
patent: 5061620 (1991-10-01), Tsikamoto
Peters et al. Cytometry 6:290-301 (1985).
Van den Engh et al. Cytometry 10:282-293 (1989).
Visser in Flow Cytometry and Sorting 1990 Wiley-USS, Inc. pp. 669-683.
Reading et al. Blood 84:399a 1994.
Lidmo et al. in Flow Cytometry and Sorting 1990 Wiley-USS, Inc. pp. 145-169.
Peters in Flow Cytogenetics 1989 Academic Press pp. 211-224.
Gray et al. Science 238:323-329 (1987).
Tanke et al. Tibtech 11:54-62 (1993).

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