Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Patent
1996-04-16
2000-10-17
Ludlow, Jan
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
436 63, 436 52, 436 53, 436149, 436164, 436180, 324 714, 356336, 209 31, 209 32, 209 33, 209655, 422 681, 422 8205, 422 8208, 422 8209, 422 99, 422100, 422101, G01N 118
Patent
active
061330442
ABSTRACT:
A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.
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Ludlow Jan
University of Washington
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