High speed digital imaging microscope

Details High speed digital imaging microscope High speed digital imaging microscope

1991-07-15

1993-08-03

Fields, Carolyn E.

Radiant energy

Luminophor irradiation

With ultraviolet source

2504612, 356417, 356418, G01J 3443, G01N 2164

Patent

active

052331970

ABSTRACT:
A high speed fluorescent emission imaging digital microscope has an Argon-Ion UV laser illumination source and a high speed optical filtering wheel in the illumination path to alternately select between two UV illumination wavelengths to produce fluorescent emission images at each UV wavelength. A dichroic reflector directs emission images along an image path and an galvanometer rotatable mirror exposes each image on a predetermined area of a CCD. A computer controls the rotatable mirror in response to exposure timing information from the filtering wheel. An objective lens is focused on the specimen for producing an image from a particular focal plane of the specimen. The objective lens is mounted on a counterweighted piezoelectric objective translator for changing the focal plane of the specimen image in response to control signals from the electronic processor.

REFERENCES:
patent: 4744667 (1988-05-01), Fay et al.
patent: 4859063 (1989-08-01), Fay et al.
patent: 5053626 (1991-10-01), Tillotson
patent: 5149972 (1992-09-01), Fay et al.
Lasser-Ross, et al., "High time resolution fluorescence imaging with a CCD camera," Journal of Neuroscience Methods, 36 (1991), pp. 253-261.
O'Rourke et al, "High-Speed Digital Imaging of Cytosolic Ca.sup.2+ and Contraction in Single Cardiomyocyts," American Physiological Society, pp. H230-H242.
Linderman et al., "Charge-Coupled Device Imaging of Rapid Calcium Transients in Cultured Arterial Smooth Muscle Cells," Cell Calcium, vol. 11, pp. 131-144, (1990).

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