Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1999-06-28
2000-05-23
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435975, C12Q 137, C12Q 100
Patent
active
060664673
ABSTRACT:
Novel enzymatic methods to determine the concentration of homocysteine in biological fluids are described. In a typical embodiment of the invention, the biological fluid sample is from a patient, and the methods of the invention are useful to assess risk for cardiovascular disease. The novel methods of the invention involve use of particular homocysteinase enzymes that permit the determination of homocysteine concentrations in biological samples without interference from the concentrations of cysteine and/or of methionine that are routinely present in such samples. There is also provided a diagnostic kit for use in determining the amount of homocysteine in a biological sample comprising (a) a homocysteinase having the aforementioned characteristics, and (b) at least one reagent capable of being used to determine the amount of product formed in the homocysteinase reaction. In a further aspect, the homocysteinase is provided as a chimeric molecule that comprises amino acid subsequences derived from, or patterned on, more than one homocysteinase, and which is typically produced from a chimeric polynucleotide that encodes therefor. Additional enhancements in homocysteine assay methodology include use of the enzyme .gamma.-glutamylcysteine synthetase to further limit any interference from cysteine present in the biological samples.
REFERENCES:
patent: 4940658 (1990-07-01), Allen et al.
patent: 5438017 (1995-08-01), Allen et al.
patent: 5478729 (1995-12-01), Van Atta et al.
patent: 5631127 (1997-05-01), Sundrehagen
patent: 5827645 (1998-10-01), Sundrehagen et al.
patent: 5985540 (1999-11-01), Tan et al.
Araki, A. et al., Journal of Chromatography (1987) 422 : 43-52. Month not available.
Bagnara, A.S. et al., Molecular and Biochemical Parasitology (1996) 81 : 1-11. Month not available.
Dudman, N.P.B. et al., Clinical Chemistry (1996) 42 (12) : 2028-2032. Month not available.
Esaki, N. et al., "L-Methionine gamma-Lyase from Pseudomonas putida and Aeromonas " in Methods in Enzymology (1987) 143 : 459-465. Month not available.
Gage, D.A. et al., Nature (1997) 387 : 891-893. Month not available.
Garg, U.C., Clinical Chemistry (1997) 43(1) : 141-145. Month not available.
Gilfix, B.M. et al., Clinical Chemistry (1997) 43(4) : 687-688. Month not available.
Hoffman, R.M. et al., "Diagnosis and treatment of homocysteine disease using recombinant homocysteinase". 2nd International Conference on Homocysteine Metabolism, Nijmegen, Netherlands, Apr. 26-29, 1998. Netherlands Journal of Medicine 52 (Suppl) 1998. S41. ISSN: 0300-2977, XP002087823. Month not available.
Hori, H. et al., Cancer Research (1996) 56 : 2116-2122. Month not available.
Inoue, Y. et al., Applied Microbiology and Biotechnology (1993) 38 : 473-477. Month not available.
Ito, S. et al., Journal of Biochemistry (1976) 79 : 1263-1272. Month not available.
Jakubowsky, H. et al., FEBS Letters (1993) 317(3) : 237-240. Month not available.
Kang, S. et al., Annual Review of Nutrition (1992) 12 : 279-298. Month not avialable.
Kerr. R.A. Science (1997) 276 : 703-704. Month not available.
Lockwood, B. et al., Biochemical Journal (1991) 279 : 675-682. Month not available.
Markos, A. et al., FEMS Microbiology Letters (1996) 135 : 259-264. Month not available.
McCully, K.S., American Journal of Pathology (1996) 56: 111-128. Month not available.
McCully, K.S., Annals of Clinical and Laboratory Science (1993) 23(6) : 477-493. Month not available.
McCully, K.S., Annals of Clinical and Laboratory Science (1994) 24(1) : 27-59. Month not available.
McCully, K.S., Annals of Clinical and Laboratory Science (1994) 24(2) : 134-152. Month not available.
McCully, K.S., Nature Medicine (1996) 2(4) : 386-389. Month not available.
McKie, A. et al., Journal of Biological Chemistry (1998) 273 : 5549-5556. Month not available.
Mottram, J.C., Gene Bank, (Jul. 17, 1997), Accession No. AJ000486, NID g2330884; and Accession No. AJ000487, NID g2330886. Month not available.
Mudd, S.H. et al., American Journal of Human Genetics (1985) 37 : 1-31. Month not available.
Nygard, O. et al., The New England Journal of Medicine (1997) 337(4) : 230-236. Month not available.
Pennist, E., Science (1997) 276 : 705-706. Month not available.
Riley, D.E. et al., Molecular and Biochemical Parasitology (1992) 51 : 161-164. Month not available.
Robinson, K. et al., Cleveland ClinicJournal of Medicine, (1994) 6 : 438-450. Month not available.
Selhub, J. et al., New England Journal. of Medicine (1995) 332 : 286-291. Month not available.
Shipchandler, M.T. et al., Clinical Chemistry (1995) 41(7) : 991-994. Month not available.
Stampfer, M. et al, Journal of the American Medical Association (1992) 268 : 877-881. Month not available.
Tan, Y. et al., Protein Expression and Purification (1997) 9 : 233-245.
Tanaka, H. et al., Journal of Applied Biochemistry (1980) 2 : 439-444.
Tanaka, H. et al., Biochemistry (1977) 16 : 100-106. Month not available.
Thong, K-W. et al., Experimental Parasitology (1987) 63 : 143-151. Month not available.
Thong, K-W. et al., IRCS Journal of Medical Science (1985) 13 : 493-494. Month not available.
Thong, K-W. et al., IRCS Journal of Medical Science (1985) 13 : 495-496. Month not available.
Thong, K-W. et al., Molecular and Biochemical Parasitology (1987) 23 : 223-231. Month not available.
Ueland, P. et al., "Plasma Homocysteine and Cardiovascular Disease", in Atherosclerotic Cardiovascular Disease, Hemostasis and Endothelial Function, Francis, R.B. Jr., ed., 1992, pp. 183-236, Marcel Dekker, Inc, New York. Month not available.
Vilaseca, M.A. et al., Clinical Chemistry (1997) 43(4) : 690-691. Month not available.
Watanabe, K. et al., Nucleic Acids Research (1986) 14 : 4393-4400. Month not available.
Wolfe, G.V. et al., Nature (1997) 387 : 894-897. Month not available.
Yamaguchi, A. et al., Annual Report of Sapporo City Institute of Public Health (1993) 20 : 67-74. Month not available.
Zuo, X. et al., Microbiology (1995) 141 : 2637-2642. Month not available.
Han Qinghong
Tan Yuying
Tang Li
Xu Mingxu
Anticancer Inc.
Leary Louise N.
LandOfFree
High specificity homocysteine assays for biological samples does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High specificity homocysteine assays for biological samples, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High specificity homocysteine assays for biological samples will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1836011