High specific activity nucleic acid probes having target recogni

Details High specific activity nucleic acid probes having target recogni High specific activity nucleic acid probes having target recogni

1988-12-16

1994-06-28

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 536 221, 536 242, 536 253, 536 254, 935 17, 935 77, 935 78, C16Q 168, G01N 3353, C07H 2104, C12Q 168

Patent

active

053248295

ABSTRACT:
The probes of the invention are made up of a target recognition moiety and a signal generation moiety. In the first step of the preparatory method of the invention, the 5' or 3' terminus or both of the target recognition moiety is chemically altered to incorporate a reactive functionality which will allow chemical linkage of this target recognition moiety with one or both termini of the signal generation moiety of the probe. Suitable functionalities vary widely, but may, in general be described as those that allow chemical attachment of the two moieties either at the 5' or 3' end of the target recognition moiety. The two moieties should not detach from each other upon hybridization of the target moiety to the target analyte. Thus, when the probe is hybridized to target analyte, it will then carry with it, signal moiety, to label this complex, which label is then later detected.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4533628 (1985-08-01), Maas
patent: 4556643 (1985-12-01), Paau et al.
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4623618 (1986-11-01), Rokugawa
patent: 4711955 (1987-12-01), Ward et al.
patent: 4717653 (1988-01-01), Webster
patent: 4794082 (1988-12-01), Sigler et al.
patent: 4816389 (1989-03-01), Sansonetti et al.
patent: 4851331 (1989-07-01), Vary et al.
Ghosh et al. (1987) Nucleic Acids Research, vol. 15, No. 13, pp. 5353-5372.
Chu et al. (1988) Nucleic Acids Research, vol. 16, No. 9, pp. 3671-3691.
Stavriamopoulos et al. (1985) Federation Proceedings, vol. 44, No. 5, p. 1622.
Bauman, J. (1985) Acta Histochemica, Suppl.-Band XXXI, pp. 9-18.
Sigma Chemical Catalog, p. 771 (St. Louis, MO) 1987 edition.
T. Brown et al., "Modern Machine--aided Methods of Oligodeoxyribonucleotide Synthesis", in Oligonucleotides and Analogues--A Practical Approach, F. Eckstein, Editor Oxford University Press, New York, N.Y., 1991, pp. 1-24.
Van der Ploeg, M., Folia Histochemica et Cytobiologica 24:189-194 (1986).
Sodja, A. et al., Nucleic Acids Research 5:385-401 (1978).
Manning et al., Chromosoma 53:107-117 (1975).
Broker, et al., Nucleic Acids Research 5:363-384 (1978).
Manning, et al., Biochemistry 16:1364-1370 (1977).
Langer, et al., Proc. Natl. Acad. Sci., USA 78:6633-6637 (1981).
Clegg & Gerlach, J. Bacteriology 169:934-938 (1987).
Moseley, et al., J. Infectious Diseases 142:892-898 (1980).
Amarnath & Broom, Chemical Reviews 77:183-217 (1977).

Affiliated with

Also associated with

Rating

High specific activity nucleic acid probes having target recogni does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High specific activity nucleic acid probes having target recogni, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High specific activity nucleic acid probes having target recogni will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2378175