Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-08-07
2007-08-07
Chunduru, Suryaprabha (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024310, C536S024320, C536S024330
Reexamination Certificate
active
10364839
ABSTRACT:
The invention features methods that are capable of detecting single target molecules in a sample input volume of, e.g., 5 μl, and of quantifying organismal, e.g., chiamydial, DNA. Desirably, these methods employ a single tube format coupled with fluorescent detection of amplicons. This approach facilitates the application of quantitative PCR (qPCR) to microbiological diagnosis in clinical settings. The invention also features primers and probes for the detection ofChlamydia. The use of specific hybridization probes with qPCR amplification provides the ability for identification of individual species or strains of microorganisms.
REFERENCES:
patent: 6130047 (2000-10-01), Nadeau et al.
patent: 6287765 (2001-09-01), Cubicciotti
patent: 6406891 (2002-06-01), Legerski
patent: 6551778 (2003-04-01), Harvey et al.
Huang et al. Quantitative detection ofChlamydiaspp. by fluorescent PCR in the LightCycler. Biothechniques, Vo. 30, No. 1, p. 150-157, 2001.
Huang et al. Quantitative detection ofChlamydiaspp. by fluorescent PCR in the LightCycler. Biothechniques, Vo. 30, No. 1, p. 150-152, 154-157, 2001.
Schawab et al. Concentration and purification of beef extract mock elutes from water samples for the detection of Enteroviruses, Hepatitis A virus, and Norwalk virus by RT-PCR. Appl. and Environ. Microbiol., vol. 61, No. 2, pp. 531-537, 1995.
Everett et al. The ribosomal intergenic spacer and domain I of 23S rRNA gene are phylogenetic markers forChlamydiaspp. International J.Systematic Bacteriol., vol. 47, No. 2, pp. 461-473, 1997.
The Light Cycler™—The Smartest Innovation for More Efficient PCR. Biochemica 1998, No. 2, 4-7.
Apfalter et al. Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection ofChlamydia pneumoniaein Endarterectomy Specimens. J. Clin. Microbiol. 2001, 39:519-524.
Boman et al. Molecular Diagnosis ofChlamydia pneumoniaeinfection. J. Clin. Microbiol. 1999, 37:3791-3799.
DeSilva et al. Rapid Genotyping and Quantification on the LightCycler™ with Hybridization Probes. Biochemica 1998, No. 2, 12-15.
Everett et al. The Ribosomal Intergenic Spacer and Domain I of the 23S rRNA Gene Are Phylogenetic Markers forChlamydiaspp. Int. J. System. Bacteriol. 1997, 47:461-473.
Everett et al. Rapid Detection of theChlamydiaceaeand Other Families in the OrderChlamydiales: Three PCR Tests. J. Clin. Microbiol. 1999, 37:575-580.
Everett et al. Identification of Nine Species of theChlamydiaceaeUsing PCR-RFLP. Int. J. System. Bacteriol. 1999, 49:803-813.
Hecker et al. High and Low Annealing Temperatures Increase Both Specificity and Yield in Touchdown and Stepdown PCR. BioTechniques 1996, 20:478-485.
Huang, et al. Quantitative Detection ofChlamydiaspp. by Fluorescent PCR in the LightCycler. 2001, 30:150-157.
Johnson et al. Evaluation of Nucleic Acid Amplification Tests as Reference Tests forChlamydia trachomatisInfections in Asymptomatic Men. J. Clin. Microbiol. 2000, 38:4382-4386.
Smieja et al. Replicate PCR Testing and Probit Analysis for Detection and Quantitation ofChlamydia pneumoniaein Clinical Specimens. J. Clin. Microbiol. 2001, 39:1796-1801.
“High Pure PCR Template Preparation Kit,” Dec. 12, 2006, pp. 15-24 <http://www.roche-applied-science.com/prod—man/pdf/chapter2/page—15-24.pdf>.
DeGraves et al., “High-Sensitivity Quantitative PCR Platform,”Bio Techniques34:106-115 (2003).
Don et al., “‘Touchdown’ PCR to Circumvent Spurious Priming During Gene Amplification,”Nucleic Acids Research, 19:4008 (1991).
Kaltenboeck et al., “Structures of and Allelic Diversity and Relationships among the Major Outer Membrane Protein (ompA) Genes of the Four Chlamydial Species,”Journal of Bacteriology, 175:487-502 (1993).
Kaltenboeck et al., “Two-Step Polymerase Chain Reactions and Restriction Endonuclease Analyses Detect and Differentiate ompA DNA ofChlamydiaspp.,”Journal of Clinical Microbiology, 30:1098-1104 (1992).
Auburn University
Chunduru Suryaprabha
Clark & Elbing LLP
LandOfFree
High-sensitivity real-time polymerase chain reaction for... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High-sensitivity real-time polymerase chain reaction for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High-sensitivity real-time polymerase chain reaction for... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3887051