High sensitivity immunoassay for polychlorinated dibenzo-p-dioxi

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436815, G01N 33535, G01N 33547

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active

056746970

ABSTRACT:
The present invention provides a method for determining qualitatively or quantitatively the presence of polychlorinated dibenzodioxins and polychlorinated dibenzofurans in a test sample. The method includes the steps of: providing a known quantity of antibodies to the polychlorinated dibenzodioxins and polychlorinated dibenzofurans; providing a competitor that will bind to said antibodies in competition with the polychlorinated dibenzodioxins and polychlorinated dibenzofurans and having a lower affinity to said antibodies than said antibodies have to the polychlorinated dibenzodioxins and polychlorinated dibenzofurans; incubating said antibodies and said competitor in the presence of a test sample; and detecting the presence of the polychlorinated dibenzodioxins and polychlorinated dibenzofurans in the test sample. The invention also provides methods of using the competitor of the present invention to detect qualitatively or quantitatively polycyclic aromatic compounds.

REFERENCES:
patent: 4238472 (1980-12-01), Albro et al.
patent: 4798807 (1989-01-01), Vanderlaan et al.
patent: 5273909 (1993-12-01), Piasio
patent: 5384262 (1995-01-01), Latt et al.
patent: 5464746 (1995-11-01), Fino
Christoffer Rappe and Hans Rudof Buser, "Chemical Properties and Analytical Methods", Himbrough (ed.) Halogenated biphenyls, terphenyls, napththalenes, dibenzodioxins and related products, Chpt. 2, 1980, pp. 40-76.
R.E. Carlson, "Hapten vs. Competitor Design Strategies for Immunoassay Development" in Judd O. Nelson, Alexander E. Karu, Rosie B. Wong, Eds., Immunoanalysis of Agrochemicals, ACS Symposium Series No. 586, Chpt. 10, 1995, pp. 140-152.
B.K. Van Weemen and A.H.W.M. Schuurs, "Sensitivity and Specificity of Hapten Enzyme-Immunoassays", in Feldman et al., Eds., First International Symposium on Immunoenzymatic Techniques, 1976, pp. 125-133.
Sherry et al., "Use of Dimethyl Sulfoxide as Solubilization Agent in the Detection of 2,3,7,8-TCDD by Radioimmunoassay", Chemosphere 20(10-12):1409-1416 (1990).
Kerkhoven et al., "The use of recombinant-DNA techniques and hybridomas to make antibody(-fragments) that specifically recognize well defined dioxins and dibenzofurans in a competitive ELISA", Dioxix '93, vol. 11:127-130 (1993).
Langley et al., "Immunoprobes for Polychlorinated Dibenzodioxins: synthesis of Immunogen and Characterization of Antibodies", Food & Agric. Immunol. 4:143-151 (1992).
Bunce et al., "Development of a Rapid Screening Assay for PCDDs and PCDFs", Chemosphere 20(10-12):1417-1422 (1990).
Collier, Thomas Lee, "Synthesis of dioxins as analytical and toxicological standards", Dissertation Abstracts International vol. 51, No. 12 (Jun. 1991), p. 5870B.
Dougherty et al., "High Affinity Assay for Polychlorodibenzodioxins and Polychlorodibenzofurans: Application to Hazardous Wastes", Abstract of a presentation to the meeting of the Division of Environmental Chemistry of the Amer. Chem. Soc. (Apr. 1986).
Watkins et al., "An Immunoassay for Chlorinated Dioxins in Soils", Chemosphere 19(1-6):267-270 (1989).
Sherry et al., "The Use of Radioimmunoassay for the Detection of Polychlorinated Dibenzo-P-dioxins in Fish Samples", Chemosphere 19(1-6):255-261 (1989).
Krieger, James, "Immunoassay for organic analyses developed", Chemical and Engineering News (Mar. 30, 1987), p. 22.
Stanker et al., "Development of an Immunoassay for Chlorinated Dioxins Based on a Monoclonal Antibody and an Enzyme Linked Immunosorbent Assay (ELISA)", Chemosphere 16(8/9):1635-1639 (1987).
Stanker et al., "Monoclonal Antibodies for Dioxin: Antibody Characterization and Assay Development", Toxicology 45:229-243 (1987).
Kennel et al., "Monoclonal Antibodies to Chlorinated Dibenzo-p-Dioxins", Chemosphere 15(9-12):2007-2010 (1986).
Kennel et al., "Monoclona Antibodies to Chlorinated Dibenzo-p-Dioxins", Toxicol. and Applied Pharmacol. 82:256-263 (1986).
Chae, et al., "A Screening Assay for the Tetrachlorodibenzo-p-dioxin Trichlorodibenzo-p-dioxin as the Binding Ligand", Intern. J. Environ. Anal. Chem. 17:267-274 (1984). Methods in Enzymology 84:619-628 (1982).
Luster et al., "Radioimmunoassay for Quantitation of 2,3,7,8-Tetrachlorodibenzofuran", Anal. Chem. 52:1497-1500 (1980).
Albro et al., "A Radioimmunoassay for Chlorinated Dibenzo-p-Dioxins", Toxicol. and Applied Pharmacol. 50:137-146 (1979).
"Paracelsian, Inc. (NASDAQ:PRLN) Announces Licensing Agreement for New Analytical Approach to Assessing Dioxin With Dow Environmental", Paracelsian release dated Jan. 17, 1995.
Robert O. Harrison, Maryanne Chamerlik-Cooper, Robert E. Carlson, "Determination of PCBs in Water, Soil, Sediment, and Oil by Enzyme Immunoassay", Proceedings: 1991 EPRI PCB Seminar, Oct. 8-11, 1991, Baltimore, Maryland.
Robert O. Harrison, Robert E. Carlson, "Analysis of PCBs by Enzyme Immunoassay", 8th Annual Waste Testing and Quality Assurance Symposium, Jul. 1992, Arlington, Virginia. Transcript of presentation.
Robert O. Harrison, Robert E. Carlson, Alan J. Weiss, "Analysis of PCBs by Enzyme Immunoassay", Third International Symposium on Field Screening, Las Vegas, Nevada, Feb. 1993.
Boehringer Manheim, Opposition to European Patent 0332819, Aug. 8, 1994.
J. McKinney, et al., "Development and Reliability of a Radio Immunoassay for 2,3,7,8-tetrachlorodibenzo-P-dioxin", Chemical Abstracts 96:209 (1982).
Vanderlaan, et al., "Improvement and Application of an Immunoassay for Screening Environmental Samples for Diosin Contamination", Env. Toxicol. 7:859-870 (1988).
R. O. Harrison, et al., "On-Site Analysis of PCB's in Water by Enzyme Immunoassay", Presentation prepared for the ACS National Meeting Mar. 1994, San Diego, CA.
Menzie et al., "Exposure to Carcinogenic PAHs in the Environment", Environ. Sci. Technol. 26(7):1278-1284 (1992).
L. Smith et al., Anal. Chem., vol. 50, No. 11, pp. 1830-1841 (1984).

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