High sensitivity fluorescent single particle and single molecule

Details High sensitivity fluorescent single particle and single molecule High sensitivity fluorescent single particle and single molecule

1989-05-26

1990-12-25

Evans, F. L.

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

356417, 2504581, G01N 2164

Patent

active

049798243

ABSTRACT:
Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.

REFERENCES:
patent: 4573796 (1986-03-01), Martin et al.
patent: 4793705 (1988-12-01), Shera
Hirschfeld, T., "Optical Microscopic Observation of Single Small Molecules", Dec. 1976, vol. 15, No. 12, Applied Optics, pp. 2965-2966.
Dovichi, Norman J.; Martin, John C.; Jett, James H.; Trkula Mitchell; Keller, Richard A., Mar. 1984, Analytical Chemistry, vol. 56, No. 3, pp. 348-354.
Nguyen, Dinh C.; Keller, Richard A.; Trkula Mitchell, "Ultrasensitive Laser--Induced Fluorescence Detection in Hydrodynamically Focused Flows", Feb. 1987, J. Opt. Soc. Am. B, vol. 4, No. 2, pp. 138-143.
Mathies A. Rschard & Stryer, Lubert, "Single--Molecule Fluorescence Detection: A Feasibility Study Using Phycoerythrin", Applications of Fluorescence in the Biomedical Sciences, pp. 129-140.
Nguyen, D. C., Keller, R. A.; Jell, J. H.; Martin, J. C., "Detection of Single Molecules of Phycoerythrin in Hydrodynamically Focused Flows by Laser--Induced Fluorescence", Anal. Chem., vol. 59, No. 17, Sept. 1, 1987, pp. 2158-2161.

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