High sensitivity dyes as stains for RNA bands in denaturing gels

Details High sensitivity dyes as stains for RNA bands in denaturing gels High sensitivity dyes as stains for RNA bands in denaturing gels

1996-09-30

1998-03-24

Gorgos, Kathryn L.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204452, G01N 2726

Patent

active

057308497

ABSTRACT:
The visualization of RNA bands in denaturing electrophoresis gels is improved by staining the gels with a 2,7-diamino-10-(N,N,N',N'-tetramethyl-1,3-propanediamino)-propyl-9-phenylp henanthridine dihalide hydrohalide or a dihalide. These dyes provide effective contrast for sensitive detection without the need for extensive washing of excess dye from the gels to lower background noise.

REFERENCES:
patent: 5312921 (1994-05-01), Glazer et al.
patent: 5437980 (1995-08-01), Haugland
patent: 5582984 (1996-12-01), Bieniarz et al.
S.C. Benson, et al., "Heterodimeric DNA-Binding Dyes Designed for Energy Transfer: Stability and Applications of the DNA Complexes, " Nucleic Acids Research (1993) 21(24):5720-5726.
S.C. Benson, et al., "Heterodimeric DNA-Binding Dyes Designed for Energy Transfer: Synthesis and Spectroscopic Properties, " Nucleic Acids Research (1993) 21(24):5727-5735.
CAplus abstaract of Gruendemann et al. ("Ethidium bromide staining during denaturation with glyoxal for sensitive detection of RNA in agarose gel electrophoresis", Anal. Biochem. (1994), 216 (2), 459-61), No month available., 1994.
CAplus abstract of Zhiyuan Gong ("Improved RNBA staining in formalehyde gels", Biotechniques (1992), 12(1), 74, 76), No month available., 1992.

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