High sensitivity assays for angiotensin converting enzyme

Details High sensitivity assays for angiotensin converting enzyme High sensitivity assays for angiotensin converting enzyme

1977-11-25

1981-06-30

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 24, 435212, 435219, 424 15, 424 2, 2601125R, C12Q 138, C12Q 136

Patent

active

042763789

ABSTRACT:
A sensitive method for measuring angiotensin converting enzyme in clinical material such as serum, urine, and tissue biopsies. The method employs Hip His Leu as the substrate. The method exploits the finding that sulfate ion in the range of 0.75 through 1.1 M lowers the apparent Km for Hip His Leu from about 2.6.times.10.sup.-3 M to about 1.8.times.10.sup.-4 M. This 14-fold decrease in Km effectively permits a more sensitive assay with Hip His Leu. The assay may be fluorimetric or radioactive means to measure the presence of eznymatic reaction.

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