High sensitivity assays for angiotensin converting enzyme

Details High sensitivity assays for angiotensin converting enzyme High sensitivity assays for angiotensin converting enzyme

1979-04-04

1981-04-07

Therkorn, Ernest G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435212, 2601125R, C12Q 136

Patent

active

042606829

ABSTRACT:
A method for measuring the activity of angiotensin converting enzyme is disclosed. In this assay, benzoylphenylalanylalanylproline or a radioisotope labelled form thereof is used as the substrate for the angiotensin converting enzyme.

REFERENCES:
patent: 3832337 (1974-08-01), Ondetti
patent: 4016042 (1977-04-01), Svendsen
patent: 4057629 (1977-11-01), Miki
patent: 4115374 (1978-09-01), Ryan et al.
Dorer et al., "Kinetic Properties of Pulmonary Angiotensin-Converting Enzyme Hydrolysis of Hippurylglyclyglycine" Biochimica et Biophysica Acta, vol. 429 (1976), pp. 220-228.
Friedland et al., "A Sensitive Fluorimetric Assay for Serum Angiotensin-Converting Enzyme" Am. J. Clin. Path., vol. 66, pp. 416-424.
Cushman et al., "Spectrophotometric Assay and Properties of the Angiotensin-Converting Enzyme of Rabbit Lungs" Biochem. Pharm., vol. 20 (1971), pp. 1637-1648.

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