High sensitivity assay method

Details High sensitivity assay method High sensitivity assay method

1983-06-22

1985-12-03

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, C12Q 126, C12Q 132

Patent

active

045566348

ABSTRACT:
A high sensitivity assay method of the coenzyme cyclic assay type, eliminates the need for a separate indication reaction, by using the cycling reaction itself as the indication reaction, according to the following steps: ##STR1## the thus-formed formazan is then colorimetrically measured, to give a highly sensitive determination of the quantity of the substance to be assayed, e.g. NAD.sup.+, NADP.sup.+, malic acid or .gamma.-aminobutyric acid in human serum.

REFERENCES:
Bergmeyer-Methods of Enzymatic Analysis vol. 1, (1974) (Acad. Press) pp. 136-143 (Article by Mollering et al.).

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