Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-01-21
1994-12-20
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 935 19, 935 77, 2041801, 2041828, 204299R, 436 94, C12Q 168, C12P 1934, G01N 3300
Patent
active
053745272
ABSTRACT:
A DNA sequencing method for use in sequencing a DNA target sequence up to 300 bases, preferably up to 500 bases or greater in length, by electrophoretically separating a mixture of single-stranded DNA sequencing fragments in a capillary tube. The method employs an aqueous denaturing solution comprising between about 4 and about 7 weight percent linear polyacrylamide molecules having an average molecular weight of between about 20 and about 100 kDa. The low-viscosity of the solution allows rapid loading and reloading of such solution into the capillary tube.
REFERENCES:
patent: 5089111 (1992-02-01), Zhu et al.
patent: 5126021 (1992-06-01), Grossman
Chiari, M., et al., "Capillary electrophoresis of macromolecules in `syrupy` solutions: Facts and misfacts," Electrophoresis 13:690-697 (1992).
Cohen, A. S., et al., "Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis," Proc. Natl. Acad. Sci. USA 85:9660-9663 (1988).
Cohen, A. S., et al., "Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis," J. Chromatography 516:49-60 (1990).
Guttman, A., et al., "Analytical and Micropreparative Ultrahigh Resolution of Oligonucleotides by Polyacrylamide Gel High-Performance Capillary Electrophoresis," Anal. Chem. 62:137-141 (1990).
Heiger, D. N., et al., "Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields," J. Chromatography 516:33-48 (1990).
Huang, K. C., et al., "Capillary Array Electrophoresis Using Laser-Excited Confocal Fluorescence Detection," Anal. Chem. 64:967-972 (1992).
Huang, X. C., et al., "Capillary gel electrophoresis of single-stranded DNA fragments with UV detection," J. Chromatography 600:289-295 (1992).
Huang, X. C., et al., "DNA Sequencing Using Capillary Array Electrophoresis," Anal. Chem. 64:2149-2154 (1992).
Mathies, R. A., and Huang, X. C., "Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing," Nature 359:167-169 (1992).
Sudor, J., et al., "Pressure refilled polyacrylamide columns for the separation of oligonucleotides by capillary electrophoresis," Electrophoresis 12:1056-1058 (1991).
Swerdlow, H., and Gesteland, R., "Capillary gel electrophoresis for rapid, high resolution DNA sequencing," Nucleic Acids Research 18(6):1415-1419 (1990).
Swerdlow, H., "Capillary gel electrophoresis for DNA sequencing. Laser-induced fluroescence detection with the sheath flow cuvette," J. Chromatograhy 516:61-67 (1990).
Applied Biosystems Inc.
Dehlinger Peter J.
Grossman Paul D.
Parr Margaret
Powers Vincent M.
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