High resolution continuous flow electrophoresis

Details High resolution continuous flow electrophoresis High resolution continuous flow electrophoresis

1985-11-25

1991-12-10

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041801, 2041821, G01N 2726, B01D 5702

Patent

active

050715364

ABSTRACT:
Recycle continuous flow electrophoresis (RCFE) makes use of an electrophoresis chamber having a plurality of inlet ports and a plurality of outlet ports disposed opposite the inlet ports. The flow exiting through an outlet port is recycled to an inlet port which is displaced by a distance .DELTA. from the inlet port directly opposite the outlet port. The distance .DELTA. is calculated using various physical parameters for the separation system and electrophoretic mobilities of the solutes. Improved resolution and reduced power requirements are achieved using the system. In addition, regeneration sections can be included to eliminate dilution of the separated solutes.

REFERENCES:
patent: 4204929 (1980-05-01), Bier
patent: 4362612 (1982-12-01), Bier
Ivory et al., in Electrophoresis '83, H. Hirai ed. Walter de Gruytor & Co., Berlin, 1984.
Hannig, 3 Electrophoresis 235-243 (1982).
Gobie et al., AICHE Abstract, p. T-6, Paper 5b, Nov. 26, 1984.
Gobie et al., ACS Abstract No. 157, (1985).
Gobie et al., 1 Biotechnology Progress 60-68 (1985).

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