High resolution biosensor for in-situ microthermometry

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435968, 436172, C12Q 102

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active

056311419

ABSTRACT:
The present invention comprises a method for use of a biosensor for in-situ microthermometry. This biosensor is a substantially spherical vesicle impregnated with a dopant which varies its optical emission spectrum as a function of the local environmental temperature of the biosensor in the temperature range to be observed. In the preferred embodiment of the present invention, this dopant is the fluorescent dye 6-dodecanoyl-2-dimethylamino-naphthalene (Laurdan.TM.). This method for in-situ measurement of temperature in a biological system uses such a vesicle by introduction of the vesicle into the biological system to be measured and manipulation of the vesicle to the location where temperature is to be measured. The temperature is then calculated from the optically measured generalized polarization at that location and the known relationship between temperature and generalized polarization for the membrane of the vesicle.

REFERENCES:
patent: 5094819 (1992-03-01), Yager et al.
patent: 5366881 (1994-11-01), Singh et al.
Epand, R., Hexagonal Phase Forming Propensity Detected in Phosphilipid Bilayers with Fluorescent Probes, Biochemistry 1992 31, 1550-1554.
Abrams, S., Use of a Visible Fluorescence Dye in a Fiberoptic Sensor to Detect General Anesthetics, Proceedings of Advances in Fluorescence Sensing Technology vol. 1885, pp. 62-71 1993.

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