High-purity rebaudioside A and method of extracting same

Details High-purity rebaudioside A and method of extracting same High-purity rebaudioside A and method of extracting same

2011-04-12

2011-04-12

Jiang, Shaojia Anna (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S128000

Reexamination Certificate

active

07923541

ABSTRACT:
A process for recovering Rebaudioside A fromStevia rebaudianaBertoni plants is provided and includes the steps of sequentially extracting plant material with a first solvent to obtain a first extract, filtering the first extract and passing the first extract through a polar resin to obtain a filtrate, and purifying the filtrate to obtain a crystalline-containing material. With further purification, a product containing over 99% Rebaudioside A is obtained. The present invention is also directed toward a method of purifying stevia glycosides to obtain purified Rebaudioside A. In one embodiment, the method includes silica gel column chromatography using an solvent comprising ethyl acetate and ethanol.

REFERENCES:
patent: 4361697 (1982-11-01), Dobberstein et al.
patent: 4892938 (1990-01-01), Giovanetto
patent: 5112610 (1992-05-01), Kienle
patent: 5962678 (1999-10-01), Payzant et al.
patent: 5972120 (1999-10-01), Kutowy et al.
patent: 6126962 (2000-10-01), Chaen et al.
patent: 2003/0138538 (2003-07-01), Kitzaume et al.
patent: 2005/0233016 (2005-10-01), Hansen
patent: 2006/0083838 (2006-04-01), Jackson et al.
patent: 2006/0134292 (2006-06-01), Abelyan et al.
patent: 2006/0142555 (2006-06-01), Jonnala et al.
patent: 2007/0082103 (2007-04-01), Magomet et al.
patent: 2007/0292582 (2007-12-01), Prakash et al.
patent: 2008/0112966 (2008-05-01), Gow et al.
patent: 1024348 (1994-04-01), None
patent: 1032651 (1996-08-01), None
patent: 1078217 (2002-01-01), None
patent: 1098860 (2003-01-01), None
patent: 1132840 (2003-12-01), None
patent: 7-143860 (1995-06-01), None
patent: 2002-262822 (2002-09-01), None
Hurtado-Benavides, A., Journal of Supercritical Fluids, Countercurrent packed column supercritical CO2 extraction of olive oil. Mass transfer evaluation, (2004), vol. 28, pp. 29-35.
Ebringerova, A., Ultrasonics Sonochemistry, Ultrasonic extraction of plant materials-investigation of hemicellulose release from buckwheat hulls, (2003), vol. 10, pp. 127-133.
Kowalska, I., Desalination, Separation of anionic surfactants on ultrafiltration membranes, (2004), vol. 162, pp. 33-40.
Koch Membrane Systems (KMS), Ultrafiltration-Filtration Overview: http://web.archive.org/web/20061106072826/www.kochmembrane.com/sep—uf.html.
Teich, L., Bioorganic and Medicinal Chemistry, Synthesis and biological evaluation of new derivatives of emodin, (2004), vol. 12, pp. 5961-5971.
Colorado University (Boulder), Organic Chemistry Undergraduate Courses, Lab Techniques: http://web.archive.org/web/20060908105657/orgchem.colorado.edu/hndbksupport/colchrom/colchrom.html.
Massachusetts Institute of Technology (MIT), Flash Column Chromatography Guide: http://ocw.mit.edu/NR/rdonlyres/Chemistry/5-301January—IAP-2004/768356CA-FD2C-4CB7-B87A-27D12504BBA8/0/8—9—flash—column.pdf.
Rohm and Haas Ion Exchange Resins, Industrial Process; Brine Softening: http://web.archive.org/web/20061105062308/www.amberlyst.com/brine—softening.htm.
Byers, John A., Solvent Polarity and Miscibility, also available at http://www.chemical-ecology.net/java/solvents.htm; or www.Phenomenex.com; published 2003; last viewed Mar. 10, 2010.
Joint FAO/WHO Expert Committee on Food Additives (JECFA), 63rd Meeting, Geneva, Italy Jun. 8-17, 2004 (5 pages).
Koyama, E., et al., “In vitro metabolism of the glycosidic sweeteners, stevia mixture and enzymatically modified stevia in human intestinal microflora”, Food and Chemical Toxicology, 41, (2005) pp. 359-374.

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