Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1990-10-23
1992-05-26
Brown, Johnnie R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 54, 536 551, 536 552, 536 553, A61K 3172.5, C08B 3710
Patent
active
051169630
ABSTRACT:
A process for preparing dermatan sulphate (DS) of pharmaceutical purity from animal organs rich in micropolysaccharides (MPS), by: a) stabilizing the fresh organs by freezing them either as such or in the form of powder, b) micronizing the material containing the MPS with an aqueous CaCl.sub.2 solution, c) digesting the homogenate comprising the raw material and the CaCl.sub.2 with proteolytic enzyme at alkaline pH and at low temperature, d) acidifying, heating and filtering the lysate, e) treating the filtrate with quaternary ammonium salts able to undergo complexing with and thus precipitate either the DS alone or all the MPS selectively, f) recovering and purifying the DS either directly from the ammonium salt complex containing it or from the complex obtained from the mixture of ammonium salt complexes of all the MPS by fractional solubilization with acetone.
REFERENCES:
patent: 3862003 (1975-01-01), Okuyama et al.
patent: 4783447 (1988-11-01), Del Bono et al.
Buddecke, Eckhart and Rudolf Drzeniek (1962), Hoppe-Scyler's Zeitschrift f. physiol. Chemie, 327:49 (abstract).
Dheu-Andries, Marie L. and Serge Perez (1983) Carbohydrate Research, 124: 324-332.
Indian Journal of Biochemistry & Biophysics, vol. 12, Sep. 1975, pp. 269-272, V. N. Satakopan et al., "Extraction of rat skin proteglycans with inorganic salts solutions".
The Methodology of Connective Tissue Research, Chapter 14, pp. 137-146; H. W. Stuhlsatz et al.: "The preparation of dermatan sulphate".
Hook et al. (1974) Biochem. J. pp. 33-43.
De Ambrosi Luigi
Del Bono Rinaldo
Ferrari Gianni
Pagella Pier G.
Rugarli Pier L.
Brown Johnnie R.
Kunz Gary L.
Mediolanum Farmaceutici Srl
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