Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2008-04-07
2010-11-09
Noakes, Suzanne M. (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C435S212000, C435S069700
Reexamination Certificate
active
07829681
ABSTRACT:
Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
REFERENCES:
patent: 6489450 (2002-12-01), Randolph et al.
Seefeldt et al. 2009, Application of High Hydrostatic Pressure to Dissociate Aggregates and Refold Protein, Current Pharmaceutical Biotechnology, 2009, vol. 10, pp. 447-555.
Shoner et al. Reconstitution of functional nuclear receptor proteins using high pressure refolding. Molecular Genetics and Metabolism, 2005, vol. 85, pp. 318-322.
Carpenter John F.
Hesterberg Lyndal K.
Randolph Theodore W.
Seefeldt Matthew B.
Barofold Inc.
Cooley LLP
Noakes Suzanne M.
LandOfFree
High-pressure inclusion body solubilization and protease... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High-pressure inclusion body solubilization and protease..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High-pressure inclusion body solubilization and protease... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4247896