Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1997-02-28
1999-08-17
Redding, David A.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204601, 204605, G01N 2726
Patent
active
059389052
ABSTRACT:
A high-pressure electrophoresis apparatus is formed by a high-pressure tubing (101) in which at least one column-shaped cell is arranged, said cell comprising two cell portions being arranged in the operating condition one above the other which form a cathode buffer solution reservoir (12) and an anode buffer solution reservoir (123) separated by a supporting member (122) which supports a carrier column loadable with a separating gel. Following implementation of high-pressure electrophoresis the carrier gel is extruded from the carrier column and subjected to further electrophoresis at normal pressure implemented on a slab gel.
REFERENCES:
patent: 5292420 (1994-03-01), Nakanura
patent: 5458761 (1995-10-01), Kamahori et al.
Electrophoresis at elevated hydrostatic pressure of the multiheme hydroxyine oxidoreductase, Masson et al, Electrophoresis, 1990, 11, pp. 128-133.
Hydrophobic interaction electrophoresis under high hydrostatic pressure: Study of the effects of pressure upon the interaction of serum albumin with a long-chain aliphatic ligand, Masson et al, Electrophoresis, 1988, 9, pp. 157-161.
Plurality of Protein Conformations of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Monomers Probed by High Pressure Electrophoresis, Erijman et al, The Journal of Biological Chemistry, vol. 268, No. 34, Issue of Dec. 5, 1993, pp. 25914-25919.
Analysis of Dissociation and Unfolding of Oligomeric Proteins Using a Flat Bed Gel Electrophoreseis at High Pressure, Paladini et al, Analytical Biochemistry 218, 1994, pp. 364-369.
Proteins under pressure, Gross et al., Eur. J. Biochem., pp. 617-630, Feb. 1994.
Slab Gel Electrophoresis of Oligomeric Proteins under High Hydrostatic Pressure, Paladini et al, Analytical Biochemistry, 1987, pp. 358-364.
Pressure Stability of Proteins, Silva et al, Annu. Rev. Phys. Chem., 1993, 44:89-113.
Clegg Robert
Erijman Leonardo
Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.v.
Redding David A.
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