High performance Zimm chromatography--HPZC

Details High performance Zimm chromatography--HPZC High performance Zimm chromatography--HPZC

1991-04-11

1992-07-14

Rosenberger, Richard A.

Optics: measuring and testing

For size of particles

By particle light scattering

356343, G01N 2151

Patent

active

051297230

ABSTRACT:
A method is disclosed based on a single injection into a modified HPLC line by which means the light scattering data required to characterize the injected molecular suspension in terms of its weight-average molecular weight, z-average square radius, and second virial coefficient may be measured and collected. The method requires that a molecular suspension at a fixed concentration be prepared and injected into a flowing stream of pure solvent following standard liquid chromatographic procedures. The sample is then passed directly to a mixing chamber whose volume is preferably several times that of the injected volume. After this mixing, the sample enters a light scattering detector where its absolute scattering intensity as a function of angle is measured for each eluting fraction. From the data so-recorded, the concentration of each fraction may be calculated and a Zimm plot may be made to yield the required molecular parameters.

REFERENCES:
patent: 3420609 (1969-01-01), Kozawa
patent: 3659946 (1972-05-01), Kozawa et al.
patent: 4565446 (1986-01-01), Chu

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