High performance microcapillary gel electrophoresis

Details High performance microcapillary gel electrophoresis High performance microcapillary gel electrophoresis

1986-10-21

1989-09-12

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2728, G01N 2726

Patent

active

048657069

ABSTRACT:
A microcapillary column for high performance electrophoresis includes a fused silica microcapillary, a gel of crosslinked polyacrylamide polymerized in the tube, and a thin layer of connecting material covalently bonded to the inner surface of the microcapillary wall and to the polymeric gel. The gel-containing microcapillary is prepared by first covalently bonding a suitable bifunctional reagent to the inner surface of the microcapillary wall, and then causing a mixture of monomer, crosslinking agent, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix which is covalently bonded to the microcapillary wall via the bifunctional reagent. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates within separation times of less than thirty minutes, and permits trace level determinations of molecular weights.

REFERENCES:
patent: 4284491 (1981-08-01), Vesterberg
patent: 4533307 (1985-08-01), Ansorge
Stellam Hjerten "High Performance Electrophoresis: The Electrophoretic Counterpart of High-Performance Liquid Chromatography", J. of Chromatography, 270 (1983) 1-6.

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