Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...
Patent
1988-12-08
1990-03-20
Therkorn, Ernest G.
Liquid purification or separation
Processes
Liquid/liquid solvent or colloidal extraction or diffusing...
210656, 210542, 530305, 530371, 530399, 530417, B01D 1508
Patent
active
049099418
ABSTRACT:
There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing 0.005 to 1M of a physiologicially acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% by volume of a physilogically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA and recombinant deoxyribonucleic proteins using reversed phased PHLC is also described.
REFERENCES:
patent: 3907676 (1975-09-01), Jorgensen
patent: 4073725 (1978-02-01), Takeuchi
patent: 4122094 (1978-10-01), Woziwodzki
patent: 4133753 (1979-01-01), Takeuchi
patent: 4289690 (1981-09-01), Pestka et al.
patent: 4377482 (1983-03-01), Rivier
patent: 4447328 (1984-05-01), Kamiyama
patent: 4485017 (1984-11-01), Tan
patent: 4499014 (1985-02-01), Estis
patent: 4500431 (1985-02-01), Miyanaga
patent: 4525465 (1985-06-01), Someno
patent: 4530963 (1985-07-01), DeVoe
patent: 4533494 (1985-08-01), Uchiyama et al.
patent: 4539399 (1985-09-01), Armstrong
patent: 4549965 (1985-10-01), Davis
patent: 4585559 (1986-04-01), DeVoe
patent: 4599175 (1986-07-01), Yamamizu
patent: 4626416 (1986-12-01), Devoe
patent: 4666604 (1987-05-01), Vijayalakshmi
patent: 4673733 (1987-06-01), Chandra
Regnier, Science, High Performance Liquid Chromatography of Biopolymers 222: 245-252, 1983.
Hancock, et al., HPLC Analysis of Biological Compounds-A Laboratory Guide, 1984.
Knighton, et al., Journal of Chromatography, 249:193-198, 1982.
Hancock, CRC Handbook of HPLC for the Separation of Amino Acids, Peptides and Proteins, II: 24,26,45, 1984.
Bishop et al., Journal of Liquid Chromatography, The Preparative Separation of Synthetic Peptides on Reversed-Phas Silica Packed in Radially Compressed Flexible-Willed Columns; 4(4):661-680, 1981.
Journal of Chromatography, Hancock, et al., Separation of Apolipproteins, 216:285-293, 1981.
Journal of Chromatography, Hancock, et al., Notes, 208:141-147, 1981.
Int. J. Peptide Protein Res., Hancock, et al., HPLC of PYR-HIS-GLY, 18:214-220, 1981.
Journal of Chromatography, Hancock, et al., Notes, 192:222-227, 1980.
McGraw-Hill Encyclopedia of Science & Technology vol. 3 at p. 104, McGraw-Hill Book Company, New York (1977).
Hancock William S.
Harding David R. K.
Poll Dick J.
LandOfFree
High performance liquid chromatography mobile phase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with High performance liquid chromatography mobile phase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High performance liquid chromatography mobile phase will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-791053