Liquid purification or separation – With means to add treating material – Chromatography
Patent
1996-06-11
1998-06-30
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
3105021, 310656, B01D 1508
Patent
active
057728747
ABSTRACT:
A method of and apparatus for liquid chromatography at improved analytical and preparative speeds and quantities involves flow of fluids through the column at flow rates sufficient to induce turbulent flow in those fluids. In one embodiment, the apparatus and method includes a substantially uniform chromatography column created by packing together a multiplicity of rigid, solid, porous particles having diameters of not less than about 30 .mu.m, surfaces of the particles being chromatographically active. A fluid mixture containing at least one solute that is reactive with the particle surfaces is injected into the column and subsequently eluted therefrom by a eluant fluid flow, both the injection and elution being effected at a velocity sufficient to induce flow within at least a substantial portion of the interstitial volume between the particles at a reduced velocity greater than about 5,000.
REFERENCES:
patent: 3493497 (1970-02-01), Pretorius et al.
patent: 4208284 (1980-06-01), Pretorius
patent: 4389385 (1983-06-01), Ramsay
patent: 4512897 (1985-04-01), Crowder
patent: 4970002 (1990-11-01), Ando et al.
patent: 5015576 (1991-05-01), Nilsson
patent: 5019270 (1991-05-01), Afeyan
patent: 5164090 (1992-11-01), Hirth
patent: 5228989 (1993-07-01), Afeyan et al.
patent: 5256298 (1993-10-01), Powell
patent: 5268097 (1993-12-01), Girot
patent: 5328603 (1994-07-01), Velander
patent: 5384042 (1995-01-01), Afeyan et al.
patent: 5387347 (1995-02-01), Rothchild et al.
patent: 5401415 (1995-03-01), Rauh
patent: 5503933 (1996-04-01), Afeyan
Snyder, Introduction to Modern Liquid Chromatography, John Wiley & Sons, 1979, pp. 173-176 and 210-216.
Guiochon, Fundamentals of Preparative and Non-Linear Chromatography, Academic Press (1984), pp. 174-176.
Kirkland, Introduction to Modern Liquid Chromatography John Wiley & Sons, 1979, pp. 234-240.
Golay, Gas Chromatography, Buttersworth, London, 1958 pp. 36-55.
Aris, Proc. Roy. Soc. A 235, 1956, pp. 67-77.
Aris, Proc. Roy. Soc A 252, 1959, pp. 538-550.
C. Giddings, Advances in the Theory of Plate Height in Gas Chromatography, Analytical Chemistry, vol. 35, No. 4, Apr. 1963, pp. 439-448.
Sir G. Taylor, Fluid Flow in Regions Bounded by Porous Surfaces, Proc. Royal Soc. of London, vol. 234A, 1956, 456-475.
W. Kopaciewicz et. al., High Velocity Reversed Phase Chromatograph of Proteins and Peptides; Use of Conventional C18, 300.ANG., 15 .mu.m Particles, J. Chromatog. A, 690, 1995, 9-19.
C. Giddings,et. al., Plate Height in Gas Chromatography,Analytical Chemistry, vol. 32, No. 7, Jun. 1960, pp. 867-870.
Introduction to Liquid Chromatography, 2nd Ed., Snyder and Kirkland, John Wiley & Sons, N.Y. (1979), pp. 238-241.
V. Pretorius and T. Smuts, Turbulent Flow Chromatography: a New Approach to Faster Analysis, Analytical Chemistry, vol. 38, No. 2, Feb. 1966, pp. 274-280).
D.S. Horne et. al., A Comparison of Mobile-Phase Dispersion in Gas and Liquid Chromatography, Sep. Science, 1(5), 531-554 (1966).
H. Kaizuma et. al., Evaluation of Coupling and Turbulence by the Dynamical Comparison of Gas and Liquid Chromatography, J. Chrom. Science, vol. 8, 630-534 (Nov. 1970).
S. H. Sumpter, et. al., Enhanced Radial Dispersion In Open Tubular Column Chromatography, J. Microcol., 3, 91-113, (1991).
H. Bauer, Open-Tubular Liquid Chromatography under Turbulent and Secondary Flow Conditions, Chromatographia, vol. 27, No. 5/6, 238-242, (Mar. 1989).
R. Tijssen, Liquid Chromatography in Helically Coiled Open Tubular Columns, Sep. Science and Tech., 13(8), 681-721 (1978).
K. Hofmann et. al., Mass Transfer in Ideal and Geometrically Deformed Open Tubes, I. Ideal and Coiled Tubes with Circular Cross-Section, J. Chromatogr. 173, 211-229 (1979).
I. Halasz, Mass Transfer in Ideal and Geometrically Deformed Open Tubes-II. Potential Applications of Ideal and Coided Tubes in Liquid Chromatography., J. Chromatogr. 173, 229-247 (1979).
M. Martin et. al., Influence of Retention on Band Broadening in Turbulent Flow Liquid and Gas Chromatography., Anal. Chem., 54, 1533-1540 (1982).
D. Dewaele et. al., Some L.C.Experiments with Capillary Columns, J. High Res. Chromatog. and Chromatog. Commun., 1, 174-176 (Sep. 1978).
K. Hofmann et. al., Mass Transfer in Ideal and Geometrically Deformed Open Tubes, III. Deformed Metal and Plastic Tubes. J. Chromatog. 199, 3, (1980).s
Quinn Hubert M.
Takarewski, Jr. Joseph J.
Cohesive Technologies Inc.
Therkorn Ernest G.
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