High performance electrophoretic mobilization of isoelectrically

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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2041829, G01N 2726

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active

047253436

ABSTRACT:
Protein zones which have been isoelectrically focused in a separation medium are mobilized for analytical or preparative purposes by changing the anolyte, catholyte or both to provide these two solutions with pH's varying in the same direction from the isoelectric points of each of said protein zones, then applying a voltage between them. The focused protein zones then elute as a unit from the separation medium while substantially maintaining their same positions relative to each other without hydrodynamic flow through the medium itself. Mobilization is alternatively achieved by hydrodynamic flow, for instance by pumping the focused protein zones with voltage maintained to suppress zone distortion (due to the hydrodynamic flow) and zone broadening (due to diffusion).

REFERENCES:
patent: 3692654 (1972-09-01), Svendsen
Boddin, M. Hilderson, H. J. Lagrou, A. Dierick; W. "Preperative Isoelectric Focusing-Eluction of the Gradient Without Interrupting the Electric Field", Analytical Biochemistry, (97, vol. 6491), pp. 293-296.
Racusen et al., "Preparative Electrophoresis on Polyacrylamide Gel," Analytical Biochemistry, 7, 62-66 (1964).
Jovin et al., "An Apparatus for Preparative Temperature-Regulated Polyacrylamide Gel Electrophoresis," Analytical Biochemistry, 9, 351-369 (1964).
Arlinger et al., "Boundary Sharpness in Capillary-Tube Isotachophoresis Demonstrated by UV Detection," Science Tools, The LKB Instrument Journal, 17, (1), 21-23 (1970).

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