High performance capillary gel electrophoresis

Details High performance capillary gel electrophoresis High performance capillary gel electrophoresis

1991-02-01

1992-02-18

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041801, G01N 2726, B01D 5702

Patent

active

050891065

ABSTRACT:
An improved, highly stable capillary column used in high performance capillary electrophoresis and method of preparing the same. The improved capillary column can significantly reduce the effects of electroosmosis during electrophoresis, is stable for reuse, and enables operation of the capillary column in high electric fields, resulting in high resolution separations in a minimum amount of time. The improved column comprises a capillary having a highly stable, coating matrix on the inner surface of the capillary wall. The highly stable coating matrix includes an intermediate layer hydrophobic phase chemically bonded to the inner wall of the capillary, a detergent which is physically adsorbed to the hydrophobic phase, and a polymerized monomer which is covalently bonded to the adsorbed detergent thereby forming a hydrophilic outer layer of the coating matrix.

REFERENCES:
patent: 3879204 (1975-04-01), Gervay
patent: 4072528 (1978-02-01), Bratt
patent: 4284518 (1981-08-01), Reick
patent: 4426489 (1984-01-01), Wessling et al.
patent: 4525526 (1985-01-01), Wessling et al.
patent: 4680201 (1987-07-01), Hjerten
patent: 4685706 (1989-09-01), Karger et al.
patent: 4690749 (1987-09-01), Van Alstine et al.
patent: 4724126 (1988-02-01), Dinbergs et al.
patent: 4770906 (1988-09-01), Harwell et al.
patent: 4791063 (1988-12-01), Hou et al.
patent: 4900627 (1990-02-01), Harwell et al.
patent: 4931328 (1990-06-01), Swedberg
patent: 4973359 (1990-11-01), Yamasoe
patent: 4981882 (1991-01-01), Smith et al.
patent: 4997536 (1991-03-01), Ohms et al.
patent: 5006313 (1991-04-01), Swedberg
patent: 5015350 (1991-05-01), Wiktorowicz
J. K. Towns et al., "Capillary Electrophoretic Separations of Proteins Using Nonionic Surfactant Coatings", Analytical Chemistry, vol. 63, No. 11 (1991), 1126-1132.
Werner G. Kuhr, "Capillary Electrophoresis", Analytical Chemistry, 62 (1990), 403R-414R.
C. J. Van Oss et al., "Cell Microelectrophoresis Simplified by the Reduction and Uniformization of the Electroosmotic Backflow", Analytical Biochemistry, 60 (1974), 242-251.
F. M. Everaerts et al., "Methods for On-Line Determination and Control of Electroendosmosis in Capillary Electrochromatography and Electrophoresis", Journal of Chromatography, 470 (1989) 89-93.
H. Poppe et al., "Capillary Zone Electrophoretic Separations of Proteins in Polyethylene Glycol-Modified Capillaries", Journal of Chromatography, 471 (1989), 429-436.
Stellan Hjerten, "High-Performance Electrophoresis: Elimination of Electroendosmosis and Solute Adsorption", Journal of Chromatography 347 (1985), 191-198.
Kelly A. Cobb, Vladislav Dolnik and Milos Novotny, "Electrophoretic Separations of Proteins in Capillaries with Hydrolytically Stable Surface Structures", Analytical Chemistry, vol. 62, No. 22 (Nov. 15, 1990), 2478-2483.
P. E. M. Verheggen, A. C. Schoots, & F. M. Everaerts, "Feasibility of Capillary Zone Electrophoresis with Suppression of Electroendosmotic Flow in Completely Closed Systems", Journal of Chromatography, 503 (1990) 245-255.
Mark Strege and Avinash Lagu, "Separation of DNA Restriction Fragments by Capillary Electrophoresis Using Coated Fused Silica Capillaries", Analytical Chemistry, vol. 63, No. 13 (Jul. 1, 1991), 1233-1236.
E. S. Huyser, "Free Radical Chain Reactions", Wiley-Interscience, pp. 154-156, N.Y. (1970).
G. Mino and S. Kaizerman, J. Polymer Sci XXX1, pp. 242-243 (1958).
E. Merk Catalog, Oct. 1989.
J. M. Neugebauer, [18] Detergents: An Overview, Methods in Enzymology, vol. 182 (1990), pp. 239-253.
M. F. Borgerding and W. L. Hinze and L. D. Stafford, GW Fulp, W. C. Hamlin, Investigations of Stationary Phase Modification by the Mobile Phase Surfactant in Micellar Liquid Chromatography, Analytical Chem. vol. 61, No. 13 (Jul. 1, 1989).
W. D. Pfeffer and Edward S. Young, Open-Tubular Liquid Chromatography with Surfactant-Enhanced Electroosmotic Flow, Analytical Chem., vol. 62, No. 20 (Oct. 15, 1990).
D. E. Keller, J. L. Torres, R. G. Carbonell, and P. K. Kilpatric, Reversible Conversion of Octadecyl-Bonded Silica to Ion-Exchange Surfaces for Protein Separations, Analytical BioChem, 176, 191-198 (1989).
J. L. Torres, R. Guzman, R. G. Carbonell, and P. K. Kilpatrick, Affinity Surfactants as Reversibly Bound Ligands for High-Performance Affinity Chromatography, Analytical BioChem 171, 441-418 (1988).
W. Muller, Eur. J. Biochem. 155 (1986), pp. 213-222.

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