High molecular weight DNA compositions for use in electrophoresi

Details High molecular weight DNA compositions for use in electrophoresi High molecular weight DNA compositions for use in electrophoresi

1990-05-15

1992-11-10

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 91, C07H 1700, C12P 1936

Patent

active

051625148

ABSTRACT:
The invention provides high molecular weight DNA length standards useful for sizing very long DNA molecules and methods for producing such standards. In a preferred embodiment, the invention provides a mixture of T4 DNA concatemers that forms a DNA "ladder" having rungs differing by at least 0.17 Mb (Megabases) in length upon gel electrophoresis and methods for producing and using such compositions.

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FUNDING: Development of the present invention was aided in part by funding from The National Institutes of Health (Grant no. GM24365). Accordingly, the Federal Government may own certain rights. Development of the invention was also facilitated by funding from the Texas Coord. Board (Grant No. 004).

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