High lysine derivatives of α-hordothionin

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

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C800S279000, C514S002600, C435S418000, C435S252300, C435S320100, C530S370000, C536S023600

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10010709

ABSTRACT:
Derivatives of α-hordothionin made by position-specific substitution with lysine residues provide lysine enrichment while retaining the antifungal activity of the parent compound.

REFERENCES:
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Altenbach et al., Manipulation of methionine-rich protein genes in plant seeds, Trends in Biotechnology (1990) 8(6):156-160.
Altenbach et al., Accumulation of a Brazil nut albumin in seed of transgenic canola results in enhanced levels of seed protein methionine, Plant Molecular Biology (1992) 18:235-245.
Anderson et al., A Transgenic Corn Line with Altered Levels of a High-Methionine Storage Protein, J Cell Biochem Suppl (1992) 16F:225 abstract Y304.
Beach et al., Enhancing the Nutritional Value of Seed Crops, Curr Top Plant Physiol: Biosynthesis and Molecular Regulation of Amino Acids in Plants (1992) 7:229-238.
Florack et al., Expression of biologically active hordothionins in tobacco. Effects of pre-and pro-sequences at the amino and carboxyl termini of the hordothionin precursor on mature protein expression and sorting, Plant Molecular Biology (1994) 24:83-96.
Garcia-Olmeda et al., Trypsin/alpha-amylase inhibitors and thionins from cereals: possible role in crop protection, Journal of Exp. Botany Supplemental (1991) 42(238):4 and abstract P1.5.
Karchi et al., Seed-specific expression of a bacterial desensitized aspartate kinase increase the production of seed threonine and methionine in transgenic tobacco, The Plant Journal (1993) 3(5):721-727.
Karchi et al., Lysine synthesis and catabolism are coordinately regulated during tobacco seed development, Proc. Natl. Acad. Sci. USA (1994) 91:2577-2581.
Maddox et al., Cloning of a barley gene alpha-hordothionin, and expression in transgenic tobacco, Journal of Cellular Biochemistry (1992) Supplement 16F, p. 217 and abstract Y212.
Rao et al., Validation of the Structure-Function Properties of Apha-Hordothionin and Derivatives Through Protein Modeling, Abstract—Advances in Gene Technology: Protein Enginering and Beyond, Miami Bio/Technology Winter Symposium (1993) vol. 3, p. 117.
Rao et al., Structure-function validation of high lysine analogs of alpha-hordothionin designed by protein modeling, Protein Engineering (1994) 7(12):1485-1493.
Florack et al., Synthetic hordothionin genes as tools for bacterial disease resistance breeding, Agricultural Biotechnology in Focus in the Netherlands (1990) pp. 39-48, Dekkers et al., eds, Pudoc, the Netherlands.
Krebbers et al., Expression of Modified Seed Storage Proteins in Transgenic Plants, Transgenic Plants (1992) pp. 37-60, Hiatt, ed., Marcel Dekker, Inc. New York.
Ponz et al., Cloning and nucleotide sequence of a cDNA encoding the precursor of the barley toxin-hordothionin, Eur. J. Biochem. (1986) 156:131-135.
Altenbach S.B. et al., “Manipulation of Methionine-Rich Protein Genes in Plant Seeds”,Trends in Biotechnology,vol. 8, No. 6, 1990 pp. 156-160.
Altenbach S.B. et al., “Accummulation of a Braxil Nut Albumin in Seed of Transgenic Canola Results in Enhanced Levels of Seed Protein Methionine”,Plant Molecular Biology,18: 235-245, 1992 pp. 235-245.
Anderson J. et al., “A Transgenic Corn Line with Altered Levels of a High-Methionine Storage Protein”,J. Cell Biochem Suppl.,vol. 16F. p. 225, 1992, abstract.
Beach, L.R. et al., “Enchancing the nutritional value of Seed Crops”,Current Top, Plant Physiol.: Biosynthesis & Molecular Regulation of Amino Acids in Plants,vol. 7, 1992 pp. 229-238.
Florack, D.E.A. et al. “Expression of biologically active hordothionin in tobacco. Effects of pre-and pro-sequences at the amino and carboxyl termini of the hordothionin precursor on mature protein expression and sorting”.Plant Molecular Biologyvol. 24 1994, pp. 83-96.
Garcia-Olmeda, F. et al. “Trypsin/alpha-amylase inhibitors and thionins from cereals: possible role in crop protection”,Journal of Exp. Botany Supplement,vol. 42. No. 238, May 1991 p. 4 and abstract p. 1.5.
Karachi H. et al., “Seed Specific Expression of a Bacterial Desensitized Aspartate Kinase Increases the Production of Seed Threonine and Methionine in Transgenic Tobacco”,The Plant Journal,vol, 3 No. 5 pp. 721-727, 1993.
Karachi H. et al., “Lysine synthesis and catabolism are coordinately regulated during tobacco seed development” PNAS 91, 1994 pp. 2577-2581. p. 2581 left column, last line right column.
Maddox, J. et al., “Cloning of a barley gene alpha-hordothionin, and expression in transgenic tobacco”J. Cell Biochem. Suppl.vol. 16F 1992 p. 217 and abstract p.212.
Rao A.G. et al., “Validation of the Structure-function properties of alpha-hordothionin and derivatives through protein modeling”-see abstract;Protein Engineering: Supplement, Advances in Gene TechnologyProtein Engineering and Beyond. Miami Winter symposium, Jam. 17-22, 1993 vol. 6, 1993.
Rao A.G. et al., “Structure-Function Validation of High Lysine Analogs of α-Hordothionin Designed by Protein Modeling”,Protein Engineering,vol. 7, No. 12, pp. 1485-1493, Dec. 1994.
Florack et al. Synthetic hordothionin genes as tools for bacterial disease resostance breeding. In: Agricultural Biotechnology in Focus in the Netherlands. Dekkers et al. eds. Pudoc. The Netherlands. pp. 39-48, 1990.
Krebbers et al. Expression of modified Seed storage proteins in transgenic plants. In:Transgenic Plants. Hiatt. ed. Marcel Dekker, Inc., New York, pp. 37-60. 1992.
Ponz et al. Cloning and nucleotide sequence of a cDNA encoding the precursor of the barley toxin -hordothionin. Eur. J. Biochem. vol., 156. pp. 131-135, 1986.

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