High loading of immobilized enzymes on activated carbon supports

Details High loading of immobilized enzymes on activated carbon supports High loading of immobilized enzymes on activated carbon supports

1977-10-03

1980-05-20

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

C07G 702

Patent

active

042040418

ABSTRACT:
Enzymes are immobilized on activated carbon supports at high load levels and with high stability by a procedure whereby a carbon support is first activated with a water-soluble carbodiimide derivative which forms a highly reactive intermediate with carboxyl and other active organic radicals on the surface of the carbon support; thereafter the complex of the carbon and carbodiimide is treated with an enzyme solution whereby the enzyme displaces the carbodiimide and forms a carbon-enzyme complex wherein the enzyme is immobilized and yet the carbon retains its surface activity. Preferred immobilizing agents are quaternary ammonium forms of aminocarbodiimides or hydrochloric acid salts of water-soluble carbodiimides. The immobilization of the enzymes on activated carbon provides materials which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

REFERENCES:
patent: 3919048 (1975-11-01), Dahlmans et al.
Liu, et al., Immobilization of Lactase on Carbon, Biotechnology and Bioengineering, vol. XVII, 1975 (pp. 1695-1696).
Line, et al., Pepsin Insolubilized by Covalent Attachment to Glass, Biochim. Biophys. Acta, vol. 242, 1971 (pp. 194-202).
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio 1973 (p. 6).

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