High loading of immobilized enzymes on activated carbon supports

Details High loading of immobilized enzymes on activated carbon supports High loading of immobilized enzymes on activated carbon supports

1980-05-07

1981-09-15

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435174, C12N 1102

Patent

active

042898536

ABSTRACT:
Enzymes are immobilized on activated carbon supports at high load levels and with high stability by reacting an activated carbon support with a carbodiimide or an isoxazolium salt to form active organic radicals on the surface of the activated carbon support and thereafter treating the carbon support with an enzyme solution to immobilize the enzyme on the carbon support. The immobilization of enzymes on activated carbon provides immobilized enzymes which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

REFERENCES:
patent: 3919048 (1975-11-01), Dahlmans et al.
patent: 4204041 (1980-05-01), Bailey et al.
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, (pp. 5-9, 24 & 25).
Liu, et al., Immobilization of Lactase on Carbon, Biotech. and Bioeng., vol. XVII, 1957, (pp. 1695-1696).
Line, et al., Pepsin Insolubilized by Covalent Attachment to Glass, Biochim. Biophys. vol. 242, 1971, (pp. 194-202).

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