High level recombinant protein production using conditional help

Details High level recombinant protein production using conditional help High level recombinant protein production using conditional help

1994-04-22

1996-05-21

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

4353201, C12N 701, C12N 1585

Patent

active

055189130

ABSTRACT:
The present invention relates to methods and compositions involving an improved recombinant transfer vector for introducing a DNA sequence, encoding a recombinant protein, into an adenovirus genome in generating recombinant adenovirus. Recombinant protein production, in cells infected with the recombinant adenovirus, can approach levels as high as 15-20% of total cellular proteins. The improved transfer vector includes an expression cassette comprising sequentially a transcription promoter, a high efficiency leader, at least one splicing signal, an enhancer-like sequence, a cloning site and a plurality of polyadenylation sites.

REFERENCES:
Lamarche et al. (1990), J. Gen. Virol. 71:1785-1792.
Leong et al. (1990), J. Virol. 64(1):51-60.
Berg et al. (1988), Nucl. Acids Res. 16(18):9057.

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