High level of expression of ingap in bacterial and euraryotic ce

Details High level of expression of ingap in bacterial and euraryotic ce High level of expression of ingap in bacterial and euraryotic ce

1997-08-12

1998-09-08

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 4353201, 536 231, 536 235, 536 241, 530350, C12N 1500

Patent

active

058044213

ABSTRACT:
Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows cultured cells to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to the cells.

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