High level of expression of INGAP in bacterial and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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C435S006120, C435S007100, C435S320100, C435S325000, C435S252300, C536S023100, C536S023500, C536S024100, C530S350000

Reissue Patent

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RE039351

ABSTRACT:
Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows cultured cells to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to the cells.

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