High level expression of immunogenic proteins in the...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S320100, C435S419000

Reexamination Certificate

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10109812

ABSTRACT:
A site specific recombination system and methods of use thereof are disclosed for manipulating the genome of higher plants. Compositions and methods for expressing immunogenic proteins using the site specific reombination system are also provided.

REFERENCES:
patent: 5571694 (1996-11-01), Makoff et al.
patent: 5614395 (1997-03-01), Ryals et al.
patent: 5686079 (1997-11-01), Curtiss, III et al.
patent: 5877402 (1999-03-01), Maliga et al.
patent: 6110736 (2000-08-01), Hodges et al.
patent: 6149919 (2000-11-01), Domenighini et al.
patent: 6297054 (2001-10-01), Maliga et al.
patent: 6376744 (2002-04-01), Maliga et al.
patent: 6388168 (2002-05-01), Maliga et al.
patent: 6472586 (2002-10-01), Maliga et al.
patent: 0 430 645 (1991-06-01), None
patent: WO 01/77353 (2001-10-01), None
Svab et al., PNAS, 1993, vol. 90, pp. 913-917.
Haq et al., Science, 1995, vol. 268, pp. 714-716.
Gianelli et al., Infect. Immun., 1997, vol. 65, pp. 331-334.
Khan, M.S. “Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants”; Nature Biotechnoogy, 17: 910-915 (1999).
Dale, E.C. “Gene transfer with subsequent removal of the selection gene from the host genome”; Proc. Natl. Acad. Sci. USA, 88: 10558-10562 (1991).
Srivastava, V. “Single-copy transgenic wheat generated through the resolution of complex integration patterns”; Proc. Natl. Acad. Sci. USA, 96: 11117-11121 (1999).
Le, Y. “Nuclear targeting determinants of the phage P1 Cre DNA recombinase”; Nucleic Acids Research, 27(24): 4703-4709 (1999).
Lyznik, LA. “Activity of yeast FLP recombinase in maize and rice protoplasts”; Nucleic Acids Research, 21(4): 969-975 (1993).
Lyznik, L.A. “FLP-mediated recombination of FRT sites in the maize genome”; Nucleic Acids Research, 24(19): 3784-3789 (1996).
Zoubenko, O.V. “Efficient targeting of foreign genes into the tobacco plastid genome”; Nucleic Acids Research, 22(19): 3819-3824 (1994).
Love, J. “Stringent control of transgene expression inArabidopsis thalianausing the Top10 promoter system”; The Plant Journal, 21(6): 579-588 (2000).
Serino, G. “A Negative selection scheme based on the expression of cytosine deaminase in plastids”; The Plant Journal, 12(3): 697-701 (1997).
Lyznik, L.A. “Heat-inducible expression of FLP gene in maize cells”; The Plant Journal, 8(2): 177-186 (1995).
Soll, J. “Protein translocation into and across the chloroplastic envelope membranes”; Plant Molecular Biology, 38: 191-207 (1998).
Adams, D. “Cre-lox Recombination inEscherichia coliCells Mechanistic Differences from the in Vitro Reaction”, J. Mol. Biol., 226: 661-673 (1992).
Craig, N.L. “The Mechanism of Conservative Site-Specific Recombination”; Annu. Rev. Genet., 22: 77-105 (1988).
Lichtenstein, C. “Prospects for reverse genetics in plants using recombination”; Plant Molecular Biology, 21: v-xii (1993).
Lubben, T.H. “Chloroplast import characteristics of chimeric proteins”; Plant Molecular Biology; 12: 13-18 (1989).
Russell, S.H. “Directed excision of a transgene from the plant genome”; Mol Gen Genet, 234: 49-59 (1992).
Timko, M.P. “Structure and Expression of Nuclear Genes Encoding Polypeptides of the Photosynthetic Apparatus”; Mol Biol of the Photosynthetic Apparatus, 381-396 (1985).
Timmermans, M.C.P. “The pFF plasmids: cassettes utilising CaMV sequences for expression of foreign genes in plants”; Journal of Biotechnology, 14: 333-344 (1990).
Wasmann, C.C. “The importance of the transit peptide and the transported protein for protein import into chloroplasts”; Mol Gen Genet, 205: 446-453 (1986).
Pizza, M. “A Genetically Detoxified Derivative of Heat-labileEscherichia coliEnterotoxin Induces Neutralizing . . . ”; J. Exp. Med., 180: 2147-2153 (1994).
Ma, S.W. “Transgenic plants expressing autoantigens fed to mice to induce oral immune tolerance”; Nature Medicine; 3(7): 793-796 (1997).
Kuroda, H. “Complementarity of the 16S rRNA penultimate stem with sequences downstream of the AUG destabilized the plastid mRNAs”; Nucleic Acids Research, 29-4: 970-975 (2001).
Kuroda, H. “Sequences Downstream of the Translation Initiation Codon Are Important Determinants of Translation Efficiency in Chloroplasts”; Plant Phys, 125: 430-436 (2001).
Ye, G. “Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco”; The Plant Journal, 25(3): 261-270 (2001).
Staub, J.M. “High-yield production of human therapeutic protein in tobacco chloroplasts”; Nature Biotechnology, 18: 333-338 (2000).
Heifetz, P.B. “Genetic engineering of the chloroplast”; Biochimie, 82: 655-666 (2000).
Giddings, G. “Transgenic plants as factories for biopharmaceuticals”; Nature Biotechnology, 18: 1151-1155 (2000).
Douce, G. “Genetically Detoxified Mutants of Heat-Labile Toxin fromEscherichia coliAre Able To Act as Oral Adjuvants”; Infection and Immunity, 67(9): 4400-4406 (1999).
Douce, G. “Mucosal immunogenicity of genetically detoxified derivatives of heat labile toxin fromEscherichia coli”; Vaccine, 16(11/12): 1065-1073 (1998).
Barchfeld, G.L. “The adjuvants MF59 and LT-K63 enhance the mucosal and systemic immunogenicity of subunit influenza vaccine administered..in mice”; Vaccine, 17: 695-704 (1999).
Carrier, H. “Kanamycin resistance as a selectable marker for plastid transformation in tobacco”; Mol Gen Genet, 241: 49-56 (1993).
Corneille, S. Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination . . . The Plant Journal, 27(2): 171-178 (2001).
Hajdukiewicz, P. “Multiple pathways for Cre/lox-mediated recombination in plastids” The Plant Journal, 27(2): 161-170 (2001).
Daniell, H. “Marker free transgenic plants: engineering the chloroplast gene without the use of antibiotic selection” Curr. Genet., 39: 109-116 (2001).
Tacket, C. “Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato” Nature Medicine, 4(5): 607-609 (1998).
Tacket, C. “A review of oral vaccination with transgenic vegetables” Microbes and Infection, 777-783 (1999).
Tregoning, J. “Expression of tetanus toxin Fragment C in tobacco chloroplasts” Nucleic Acids Research, 31(4): 1174-1179 (2003).
Bock, R. “Transgenic Plastids in Basic Research and Plant Biotechnology” J. Mol. Biol., 312: 425-438 (2001).
Magagnoli, C. “Mutations in the A Subunit Affect Yield, Stability, and Protease Sensitivity of Nontoxic Derivatives . . . ” Infection and Immunity, 64(12): 5434-5438 (1996).

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