High level expression and facile purification of proteins, pepti

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530350, 530402, 536 234, C07K 1400

Patent

active

060692308

ABSTRACT:
A process for pro and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.

REFERENCES:
patent: 4512922 (1985-04-01), Jones et al.
patent: 4743679 (1988-05-01), Cohen et al.
patent: 4952496 (1990-08-01), Studier et al.
patent: 5008373 (1991-04-01), Kingsman et al.
patent: 5302526 (1994-04-01), Keck et al.
patent: 5322769 (1994-06-01), Bolling et al.
patent: 5322930 (1994-06-01), Tarnowski et al.
Duong, Le T.; Caulfield, Michael, P., and Rosenblatt, Michael, Synthetic Signal Peptide and Analogs Display Different Activities in Mammalian and Plant in Vitro Secretion Systems, The Journal of Biological Chemistry (May 5, 1982), Baltimore, MD, USA, v262, No. 13, pp. 6328-6333.
Ngsee, Johnny K.; Hansen, William; Walter, Peter; and Smith, Michael, Cassette Mutagenic Analysis of the Yeast Invertase Signal Peptide: Effects on Protein Translocation, Molecular and Cellular Biology (Aug. 9, 1989), Washington, DC, US, v9, No. 8, pp. 3400-3410.
Randall, L.L. and Hardy, S.J.S., Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export, Science (Mar. 3, 1989), Washington, D.C., USA, v243, pp. 1156-1159.
Yamamoto, Yoshio; Taniyama, Yoshio; Kikuchi, Masakazu; and Ikehara, Morio, Engineering of the Hydrophobic Segment of the Signal Sequence For Efficient Secretion of Human Lysozyme by Saccharomyces cerevisiae; Biochemical and Biophysical Research Communications (Dec. 16, 1987), Duluth, MN, USA, v149, No. 2, pp. 431-436.
Lewin, Genes IV (1990) Oxford University Press, pp. 118-119.
Bolivar et al, 1977, Gene 2:95.
Clewell, D. R. et al, 1969, Proc Nat Acad Sci USA 62: 1159.
Cohen, S.N. et al., 1972, Proc Nat Acad Sci USA 69: 2110.
Current Protocols in Molecular Biology (1994; chapter 16).
Klein, J., 1990 Immunology, Blackwell Scientific Publications, Inc. Massachusetts, 269-293.
Kohler & Milstein, 1975, Nature, 256: 495.
Lin, S. et al., 1987, Biochemistry, 26: 5267-5274.
Sano, T. et al., 1992, Science 258: 120-122.
Smith, A. J. et al., 1992, Techniques in Protein Chemistry III, 219-229.
Studier F. W., et al, 1990, Meth. Enzym. 185: 60-89.
Tae H. J., et al., 1983, Methods in Enzymology (Chapter 51), Academic Press, Inc., 91: 580-609.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

High level expression and facile purification of proteins, pepti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High level expression and facile purification of proteins, pepti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High level expression and facile purification of proteins, pepti will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1911134

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.